Advanced Protocols for Animal Transgenesis
An ISTT Manual
Herausgeber: Pease, Shirley, Saunders, Thomas L. (Eds.)
Vorschau- Provides step-by-step instructions and helpful troubleshooting notes
- Published in cooperation with the International Society for Transgenic Technology (ISTT)
- With contributions by leading scientists
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This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.
Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
- Inhaltsverzeichnis (26 Kapitel)
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Patent and Licensing Issues in Transgenic Technology
Seiten 1-23
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Global Resources: Including Gene Trapped ES Cell Clones - Is Your Gene Already Knocked Out?
Seiten 25-42
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Designing Transgenes for Optimal Expression
Seiten 43-56
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Gene Targeting Vector Design for Embryonic Stem Cell Modifications
Seiten 57-79
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Transgenic Production Benchmarks
Seiten 81-97
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Inhaltsverzeichnis (26 Kapitel)
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Bibliografische Information
- Bibliographic Information
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- Buchtitel
- Advanced Protocols for Animal Transgenesis
- Buchuntertitel
- An ISTT Manual
- Herausgeber
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- Shirley Pease
- Thomas L. Saunders
- Titel der Buchreihe
- Springer Protocols Handbooks
- Copyright
- 2011
- Verlag
- Springer-Verlag Berlin Heidelberg
- Copyright Inhaber
- Springer-Verlag Berlin Heidelberg
- eBook ISBN
- 978-3-642-20792-1
- DOI
- 10.1007/978-3-642-20792-1
- Hardcover ISBN
- 978-3-642-20791-4
- Softcover ISBN
- 978-3-662-51943-1
- Buchreihen ISSN
- 1949-2448
- Auflage
- 1
- Seitenzahl
- XV, 669
- Anzahl der Bilder
- 145 schwarz-weiß Abbildungen, 65 Abbildungen in Farbe
- Themen
