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Basic Techniques in Molecular Biology

  • Book
  • © 2000

Overview

Authors:
  1. Stefan Surzycki

    1. Department of Biology, Indiana University, Bloomington, USA

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  • Laboratory manual with detailed step-by-step protocols
  • All of the procedures have either been used extensively in the teaching of undergraduate laboratories and adult workshops or tested in the authors laboratory
  • Each technique includes an overview of its importance and theoretical background
  • An ideal introduction to molecular biology techniques
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-VIII
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  2. General Aspects of DNA Isolation and Purification

    • Stefan Surzycki
    Pages 1-32

  3. Preparation of Genomic DNA from Animal Cells

    • Stefan Surzycki
    Pages 33-56

  4. Preparation of Genomic DNA from Plant Cells

    • Stefan Surzycki
    Pages 57-78

  5. Preparation of Genomic DNA from Bacteria

    • Stefan Surzycki
    Pages 79-100

  6. Isolation of Plasmid DNA

    • Stefan Surzycki
    Pages 101-118

  7. Isolation and Purification of RNA

    • Stefan Surzycki
    Pages 119-144

  8. Isolation of PolyA+ RNA

    • Stefan Surzycki
    Pages 145-162

  9. Agarose Gel Electrophoresis of DNA

    • Stefan Surzycki
    Pages 163-191

  10. Preparation of Probes for Hybridization

    • Stefan Surzycki
    Pages 192-220

  11. Nucleic Acid Hybridization. A Theoretical Consideration

    • Stefan Surzycki
    Pages 221-232

  12. DNA Transfer and Hybridization

    • Stefan Surzycki
    Pages 233-262

  13. Northern Transfer and Hybridization

    • Stefan Surzycki
    Pages 263-297

  14. DNA Cloning. General Consideration

    • Stefan Surzycki
    Pages 298-319

  15. DNA Cloning - Experimental Procedures

    • Stefan Surzycki
    Pages 320-373

  16. DNA Sequencing

    • Stefan Surzycki
    Pages 374-405

  17. PCR Analysis

    • Stefan Surzycki
    Pages 406-421

  18. Back Matter

    Pages 423-434
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Keywords

About this book

This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

Reviews

 

This laboratory manual differs from many of the numerous others currently on the market in that is explains the "Whys" as well as the "Hows" of the most commonly used molecular techniques. ... It is absolutely jam packed full of those little titbits of information that friendly Post Docs occasionally feel inclined to share with students. ... I liked this book, ... (it) is principally aimed at students ... and the emphasis is very definitely on making techniques accessible to these people. ... this particular book more than adequately fills the niche it is intended to cover.

Kate E. Plant, Univ. of Oxford, UK in Journal of Cell Science 2000, 113

 

Authors and Affiliations

  • Department of Biology, Indiana University, Bloomington, USA

    Stefan Surzycki

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