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Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

  • Book
  • © 2000

Overview

Editors:
  1. Thierry Rabilloud

    1. CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, Grenoble Cedex 9, France

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  • This is the first book to comprehensively cover the application of 2D gel technology to the field of proteomics
  • Includes supplementary material: sn.pub/extras

Part of the book series: Principles and Practice (PRINCIPLES)

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Table of contents (11 chapters)

  1. Front Matter

    Pages I-XVI
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  2. Introduction: The Virtue of Proteomics

    • T. Rabilloud, I. Humphery-Smith
    Pages 1-8

  3. Solubilization of Proteins in Two-Dimensional Electrophoresis

    • T. Rabilloud, M. Chevallet
    Pages 9-29

  4. Two-Dimensional Electrophoresis with Carrier Ampholytes

    • C. Monribot, H. Boucherie
    Pages 31-55

  5. Two-Dimensional Electrophoresis with Immobilized pH Gradients

    • A. Görg, W. Weiss
    Pages 57-106

  6. Detection of Proteins on Two-Dimensional Electrophoresis Gels

    • T. Rabilloud, S. Charmont
    Pages 107-126

  7. Protein Blotting and Immunoblotting

    • L. Bini, S. Liberatori, B. Magi, B. Marzocchi, R. Raggiaschi, V. Pallini
    Pages 127-141

  8. Identification of Proteins by Amino Acid Composition After Acid Hydrolysis

    • M. I. Tyler, M. R. Wilkins
    Pages 143-161

  9. Identification by Amino Acid Composition obtained from Labeling

    • J. Labarre, M. Perrot
    Pages 163-179

  10. Identification of Proteins by Amino Acid Sequencing

    • F. Lottspeich
    Pages 181-196

  11. Identification of Proteins by Mass Spectrometry

    • G. L. Corthals, S. P. Gygi, R. Aebersold, S. D. Patterson
    Pages 197-231

  12. Mass Spectrometry of Intact Proteins from Two-Dimensional PAGE

    • Ch. Eckerskorn, K. Strupat
    Pages 233-244

  13. Back Matter

    Pages 245-248
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Keywords

About this book

Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.

Editors and Affiliations

  • CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, Grenoble Cedex 9, France

    Thierry Rabilloud

Bibliographic Information

  • Book Title: Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

  • Editors: Thierry Rabilloud

  • Series Title: Principles and Practice

  • DOI: https://doi.org/10.1007/978-3-642-57105-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Hardcover ISBN: 978-3-540-65689-0Published: 17 September 1999

  • Softcover ISBN: 978-3-540-65792-7Published: 17 September 1999

  • eBook ISBN: 978-3-642-57105-3Published: 01 December 2013

  • Series ISSN: 1866-914X

  • Series E-ISSN: 1866-9158

  • Edition Number: 1

  • Number of Pages: XVI, 248

  • Topics: Proteomics, Biochemistry, general, Human Genetics, Molecular Medicine, Cancer Research

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