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- Über dieses Buch
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Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding. - Stimmen zum Buch
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From the reviews:
"In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. … It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding." (New Drugs, Issue 7, October, 2002)
"Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications … . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students." (Biotech International, Vol. 15 (2), 2003)
- Inhaltsverzeichnis (11 Kapitel)
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SPOT Synthesis — Scope of Applications
Seiten 1-22
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Chemistry of Fmoc Peptide Synthesis on Membranes
Seiten 23-39
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Manual Peptide Synthesis
Seiten 41-53
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Automated Synthesis of Solid-Phase Bound Peptides
Seiten 55-68
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Epitope Mapping of Antibodies with Solid-Phase Oligopeptides
Seiten 69-82
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Inhaltsverzeichnis (11 Kapitel)
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Bibliografische Information
- Bibliographic Information
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- Buchtitel
- Peptide Arrays on Membrane Supports
- Buchuntertitel
- Synthesis and Applications
- Herausgeber
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- Joachim Koch
- Michael Mahler
- Titel der Buchreihe
- Springer Lab Manuals
- Copyright
- 2002
- Verlag
- Springer-Verlag Berlin Heidelberg
- Copyright Inhaber
- Springer-Verlag Berlin Heidelberg
- eBook ISBN
- 978-3-662-09229-3
- DOI
- 10.1007/978-3-662-09229-3
- Softcover ISBN
- 978-3-642-07639-8
- Softcover ISBN
- 978-3-540-42532-8
- Auflage
- 1
- Seitenzahl
- X, 173
- Anzahl der Bilder
- 23 schwarz-weiß Abbildungen
- Themen
