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Separation Techniques Applied to Omics Sciences

From Principles to Relevant Applications

  • Demonstrates that a multiplatform methodology is required for a comprehensive omics analyses, and separation techniques is of outcome importance
  • The main characteristics of each separation technique suitable to each omic approach will be presented
  • First book to show relevant applications in genomics, proteomics, metabolomics and foodomics, concomitantly

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1336)

Part of the book sub series: Proteomics, Metabolomics, Interactomics and Systems Biology (PMISB)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xii
  2. The New Omics Era into Systems Approaches: What Is the Importance of Separation Techniques?

    • Flávia da Silva Zandonadi, Fábio Santos Neves, Elisa Castañeda Santa Cruz, Alessandra Sussuilini, Ana Valéria Colnaghi Simionato
    Pages 1-15
  3. Biological Applications for LC-MS-Based Proteomics

    • Bradley J. Smith, Daniel Martins-de-Souza
    Pages 17-29
  4. The Role of Chromatographic and Electromigration Techniques in Foodomics

    • Javier González-Sálamo, Diana Angélica Varela-Martínez, Miguel Ángel González-Curbelo, Javier Hernández-Borges
    Pages 31-49
  5. CE-MS for Proteomics and Intact Protein Analysis

    • Valeriia O. Kuzyk, Govert W. Somsen, Rob Haselberg
    Pages 51-86
  6. Peptidomics and Capillary Electrophoresis

    • Sille Štěpánová, Václav Kašička
    Pages 87-104
  7. Practical Considerations in Method Development for Gas Chromatography-Based Metabolomic Profiling

    • Andre Cunha Paiva, Amilton Moreira de Oliveira, Juliana Crucello, Roselaine Facanali, Leandro Wang Hantao
    Pages 139-157
  8. Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview

    • Izadora L. Furlani, Estéfane da Cruz Nunes, Gisele A. B. Canuto, Adriana N. Macedo, Regina V. Oliveira
    Pages 179-213
  9. Analytical Platforms for Mass Spectrometry-Based Metabolomics of Polar and Ionizable Metabolites

    • Adriana N. Macedo, Andrea T. Faccio, Tatiana S. Fukuji, Gisele A. B. Canuto, Marina F. M. Tavares
    Pages 215-242
  10. Metabolomics Data Treatment: Basic Directions of the Full Process

    • Hans Rolando Zamora Obando, Gustavo Henrique Bueno Duarte, Ana Valéria Colnaghi Simionato
    Pages 243-264
  11. Back Matter

    Pages 265-273

About this book

This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole.

Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biologyapproach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one. 



Editors and Affiliations

  • Analytical Chemistry Department, University of Campinas, Institute of Chemistry, Campinas, Brazil

    Ana Valéria Colnaghi Simionato

About the editor

Ana Valéria Colnaghi Simionato is an associate professor at Institute of Chemistry of Unicamp. She advises scientific initiation, masters and doctorate students as well as supervises post-doctorate researches in projects related to the investigation of biomolecules or pharmaceuticals compounds in several sample matrices, such as: culture media, blood serum, urine, saliva, medicines and meat using a metabolomics approaches. The instrumental techniques used for this purpose include capillary electrophoresis, liquid chromatography, gas chromatography and mass spectrometry. She is also part of the National Institute of Science and Technology in Bioanalytics.

Bibliographic Information

  • Book Title: Separation Techniques Applied to Omics Sciences

  • Book Subtitle: From Principles to Relevant Applications

  • Editors: Ana Valéria Colnaghi Simionato

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-3-030-77252-9

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2021

  • Hardcover ISBN: 978-3-030-77251-2Published: 10 October 2021

  • Softcover ISBN: 978-3-030-77254-3Published: 10 October 2022

  • eBook ISBN: 978-3-030-77252-9Published: 09 October 2021

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: XII, 273

  • Number of Illustrations: 13 b/w illustrations, 61 illustrations in colour

  • Topics: Systems Biology, Proteomics, Metabolomics

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access