Editors:
- Serves as a comprehensive resource to identify and characterize homing endonucleases and much more
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1123)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (17 protocols)
-
Front Matter
-
Back Matter
About this book
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.
Editors and Affiliations
-
University of Western Ontario, London, Canada
David R. Edgell
Bibliographic Information
Book Title: Homing Endonucleases
Book Subtitle: Methods and Protocols
Editors: David R. Edgell
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-968-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-62703-967-3Published: 09 February 2014
Softcover ISBN: 978-1-4939-6319-5Published: 23 August 2016
eBook ISBN: 978-1-62703-968-0Published: 08 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 284
Number of Illustrations: 21 b/w illustrations, 39 illustrations in colour
Topics: Human Genetics, Genetic Engineering