Mammalian Artificial Chromosomes

1. Front Matter

   Pages i-xi

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2. From Natural to Artificial Chromosomes

   • Vittorio Sgaramella, Sandro Eridani

   Pages 1-13

3. Methodologies Applied to Domestic Animal Chromosomes

   • Leopoldo Iannuzzi

   Pages 15-34

4. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes

   • Grzegorz Ira, Jacek Król, Jan Filipski

   Pages 35-52

5. Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals

   • Yuji Goto, Robert Feil

   Pages 53-75

6. Centromeres and Neocentromeres

   • Gérard Roizés, Christoph Grunau, Jérôme Buard, Albertina De Sario, Jacques Puechberty

   Pages 77-104

7. Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins

   • Alessandro Vindigni, Gulnara Abdurashidova, Arturo Falaschi

   Pages 105-122

8. Telomere Length Analysis and In Vitro Telomerase Assay

   • Fiorentina Ascenzioni, Pier Assunta Fradiani, Pierluigi Donini

   Pages 123-146

9. Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice

   • Peter J. Hornsby

   Pages 147-166

10. Chromosome-Based Vectors for Mammalian Cells

    • Han N. Lim, Christine J. Farr

    Pages 167-186

11. Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor

    • Jose I. de las Heras, Leonardo D’Aiuto, Howard Cooke

    Pages 187-206

12. Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis

    • Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida

    Pages 207-225

13. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice

    • Diane P. Monteith, Josephine D. Leung, Anita H. Borowski, Deborah O. Co, Tünde Praznovszky, Frank R. Jirik et al.

    Pages 227-242

14. Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications

    • Michael R. Orford, Duangporn Jamsai, Samuel McLenachan, Panayiotis A. Ioannou

    Pages 243-266

15. Back Matter

    Pages 267-269

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In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.

• Istituto di Tecnologie Biomediche Avanzate, Consiglio Nationale Ricerche, Segrate, Italy

  Vittorio Sgaramella, Sandro Eridani

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