Overview
- Offers over 200 key, readily reproducible protocols that ensure robust, successful results
- Includes many new techniques for the study of post-translational modifications
- Demonstrates the range of techniques available and their strengths and limitations
- Contains numerous time-saving techniques for even the most highly skilled researchers
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (208 protocols)
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Front Matter
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Quantitation of Proteins
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Electrophoresis of Proteins and Peptides and Dectection in Gels
Keywords
About this book
Since the publication of the bestselling second edition of John Walker’s widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results
Editors and Affiliations
Bibliographic Information
Book Title: The Protein Protocols Handbook
Editors: John M. Walker
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-1-59745-198-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-880-5Published: 07 October 2009
Softcover ISBN: 978-1-60327-474-6Published: 07 October 2009
eBook ISBN: 978-1-59745-198-7Published: 09 June 2010
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 3
Number of Pages: LXX, 1984