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Histidine Phosphorylation

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Claire E. Eyers

    1. Department of Biochemistry, Centre for Proteome Research Institute of Integrative Biology, University of Liverpool, Liverpool, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2077)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems

    • Felipe Trajtenberg, Alejandro Buschiazzo
    Pages 1-18

  3. A High-Throughput Strategy for Recombinant Protein Expression and Solubility Screen in Escherichia coli : A Case of Sensor Histidine Kinase

    • Agnieszka Szmitkowska, Blanka Pekárová, Jan Hejátko
    Pages 19-36

  4. SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation

    • Jonathan T. Fischer, Ilana Heckler, Elizabeth M. Boon
    Pages 37-49

  5. A Quantitative Method for the Measurement of Protein Histidine Phosphorylation

    • Paul V. Attwood
    Pages 51-61

  6. Analysis of 1- and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria

    • Alice K. M. Clubbs Coldron, Dominic P. Byrne, Patrick A. Eyers
    Pages 63-81

  7. Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry

    • Lauren J. Tomlinson, Alice K. M. Clubbs Coldron, Patrick A. Eyers, Claire E. Eyers
    Pages 83-91

  8. Protein Phosphohistidine Phosphatases of the HP Superfamily

    • Daniel J. Rigden
    Pages 93-107

  9. In Vitro Assays for Measuring Protein Histidine Phosphatase Activity

    • Brandon S. McCullough, Amy M. Barrios
    Pages 109-120

  10. Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases

    • Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, Patricia Casino
    Pages 121-140

  11. Manipulation of Bacterial Signaling Using Engineered Histidine Kinases

    • Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, W. Seth Childers
    Pages 141-163

  12. Development of a Light-Dependent Protein Histidine Kinase

    • Aleksandra E. Bury, Klaas J. Hellingwerf
    Pages 165-180

  13. Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting Using pHis mAbs

    • Rajasree Kalagiri, Kevin Adam, Tony Hunter
    Pages 181-191

  14. Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections

    • Natalie Luhtala, Tony Hunter
    Pages 193-208

  15. Subcellular Localization of Histidine Phosphorylated Proteins Through Indirect Immunofluorescence

    • Kevin Adam, Tony Hunter
    Pages 209-224

  16. High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry

    • Gemma Hardman, Claire E. Eyers
    Pages 225-235

  17. Proteome Bioinformatics Methods for Studying Histidine Phosphorylation

    • Andrew R. Jones, Oscar Martin Camacho
    Pages 237-250

  18. Back Matter

    Pages 251-256
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Keywords

About this book

This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field. 

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Editors and Affiliations

  • Department of Biochemistry, Centre for Proteome Research Institute of Integrative Biology, University of Liverpool, Liverpool, UK

    Claire E. Eyers

About the editor

 

Bibliographic Information

  • Book Title: Histidine Phosphorylation

  • Book Subtitle: Methods and Protocols

  • Editors: Claire E. Eyers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9884-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9883-8Published: 10 November 2019

  • Softcover ISBN: 978-1-4939-9886-9Published: 10 November 2020

  • eBook ISBN: 978-1-4939-9884-5Published: 09 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 256

  • Number of Illustrations: 10 b/w illustrations, 44 illustrations in colour

  • Topics: Biochemistry, general

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