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Transgenic Mouse

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Melissa A. Larson

    1. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA, Kansas City, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2066)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. The History of Transgenesis

    • Thomas L. Saunders
    Pages 1-26

  3. Pronuclear Microinjection of One-Cell Embryos

    • Melissa A. Larson
    Pages 27-33

  4. Integrase-Mediated Targeted Transgenics Through Pronuclear Microinjection

    • Ruby Yanru Chen-Tsai
    Pages 35-46

  5. In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos

    • Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian
    Pages 47-57

  6. Targeted Mutations in the Mouse via Embryonic Stem Cells

    • Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, Lauryl M. J. Nutter
    Pages 59-82

  7. Blastocyst Microinjection with Embryonic Stem Cells

    • Melissa A. Larson
    Pages 83-88

  8. Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos

    • Bin Gu, Marina Gertsenstein, Eszter Posfai
    Pages 89-100

  9. Embryo Transfer Surgery

    • Melissa A. Larson
    Pages 101-106

  10. Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device

    • Barbara J. Stone
    Pages 107-111

  11. Nuclear Transfer and Cloning

    • Ling Liu
    Pages 113-124

  12. Sperm-Mediated Genetic Modifications

    • Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni
    Pages 125-132

  13. Genotyping Genetically Modified (GM) Mice

    • Neeraj K. Aryal, Jan Parker-Thornburg
    Pages 133-148

  14. Nomenclature: Naming Your Gene-Modified Mouse

    • Alicia Valenzuela
    Pages 149-162

  15. Breeding Strategies for Genetically Modified Mice

    • Jan Parker-Thornburg
    Pages 163-169

  16. Strategies for Behaviorally Phenotyping the Transgenic Mouse

    • Kenneth E. McCarson
    Pages 171-194

  17. Cryobanking and Recovery of Genetically Modified Mice

    • Toru Takeo, Naomi Nakagata
    Pages 195-209

  18. Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques

    • Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata
    Pages 211-216

  19. Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus

    • Julia M. Draper, Jay L. Vivian
    Pages 217-234

  20. Back Matter

    Pages 235-236
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Keywords

About this book

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. 


Editors and Affiliations

  • Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA, Kansas City, USA

    Melissa A. Larson

Bibliographic Information

  • Book Title: Transgenic Mouse

  • Book Subtitle: Methods and Protocols

  • Editors: Melissa A. Larson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9837-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9836-4Published: 12 September 2019

  • Softcover ISBN: 978-1-4939-9839-5Published: 12 September 2020

  • eBook ISBN: 978-1-4939-9837-1Published: 11 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 236

  • Number of Illustrations: 18 b/w illustrations, 27 illustrations in colour

  • Topics: Human Genetics, Animal Models, Stem Cells

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