Skip to main content
SpringerLink
Log in
Book cover

Trinucleotide Repeats

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Guy-Franck Richard

    1. Department Genomes & Genetics, Institut Pasteur, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2056)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Experimenting with Trinucleotide Repeats: Facts and Technical Issues

    • Guy-Franck Richard
    Pages 1-10

  3. Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions

    • Stéphanie Tomé, Geneviève Gourdon
    Pages 11-23

  4. Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae

    • Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, Jennifer A. Surtees
    Pages 25-68

  5. Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis

    • David Viterbo, Guy-Franck Richard
    Pages 69-81

  6. Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae

    • Erica J. Polleys, Catherine H. Freudenreich
    Pages 83-101

  7. Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae

    • Wenying Guo, Kirill S. Lobachev
    Pages 103-112

  8. Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay

    • Lucie Poggi, Bruno Dumas, Guy-Franck Richard
    Pages 113-120

  9. Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site

    • Rujuta Yashodhan Gadgil, S. Dean Rider Jr., Todd Lewis, Joanna Barthelemy, Michael Leffak
    Pages 121-136

  10. Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells

    • Artem V. Kononenko, Thomas Ebersole, Sergei M. Mirkin
    Pages 137-150

  11. Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture

    • Gregory M. Williams, Robert S. Lahue
    Pages 151-172

  12. Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model

    • Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, Karen Usdin
    Pages 173-186

  13. In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA

    • Remco T. P. van Cruchten, Derick G. Wansink
    Pages 187-202

  14. FISH Protocol for Myotonic Dystrophy Type 1 Cells

    • Arnaud F. Klein, Ludovic Arandel, Joelle Marie, Denis Furling
    Pages 203-215

  15. Real Time Videomicroscopy and Semiautomated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases

    • Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, Mário Gomes-Pereira
    Pages 217-240

  16. Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia

    • Olivier Griso, Hélène Puccio
    Pages 241-253

  17. GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats

    • Cinzia Cinesi, Bin Yang, Vincent Dion
    Pages 255-268

  18. Gene Therapy for Huntington’s Disease Using Targeted Endonucleases

    • Magdalena Dabrowska, Marta Olejniczak
    Pages 269-284

  19. Back Matter

    Pages 285-286
    Download chapter PDF
Back to top

Keywords

About this book

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Editors and Affiliations

  • Department Genomes & Genetics, Institut Pasteur, Paris, France

    Guy-Franck Richard

Bibliographic Information

  • Book Title: Trinucleotide Repeats

  • Book Subtitle: Methods and Protocols

  • Editors: Guy-Franck Richard

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9784-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9783-1Published: 05 October 2019

  • Softcover ISBN: 978-1-4939-9786-2Published: 05 October 2020

  • eBook ISBN: 978-1-4939-9784-8Published: 04 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 286

  • Number of Illustrations: 12 b/w illustrations, 53 illustrations in colour

  • Topics: Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation