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Bioconjugation

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sam Massa

    1. Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Nick Devoogdt

    1. In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2033)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Site-Specific Conjugation of Thiol-Reactive Cytotoxic Agents to Nonnative Cysteines of Engineered Monoclonal Antibodies

    • Stephan Dickgiesser, Roland Kellner, Harald Kolmar, Nicolas Rasche
    Pages 1-14

  3. Application of Next-Generation Maleimides (NGMs) to Site-Selective Antibody Conjugation

    • Maurício Morais, Nafsika Forte, Vijay Chudasama, James R. Baker
    Pages 15-24

  4. Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent

    • Maria J. Matos, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes
    Pages 25-37

  5. Engineering Dual Variable Domains for the Generation of Site-Specific Antibody–Drug Conjugates

    • Alex R. Nanna, Christoph Rader
    Pages 39-52

  6. Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutaminase

    • Jared L. Spidel, Earl F. Albone
    Pages 53-65

  7. Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine

    • Rania A. Hashad, Jaclyn L. Lange, Natasha C. W. Tan, Karen Alt, Christoph E. Hagemeyer
    Pages 67-80

  8. CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow for Site-Specific Conjugation

    • Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, Vladimir R. Muzykantov
    Pages 81-93

  9. Site-Specific Modification of Proteins via Trypsiligase

    • Sandra Liebscher, Frank Bordusa
    Pages 95-115

  10. Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation

    • Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers et al.
    Pages 117-130

  11. Site-Specific Bioconjugation Using SMARTag® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody–Drug Conjugates

    • Junjie Liu, Robyn M. Barfield, David Rabuka
    Pages 131-147

  12. Protein Labeling and Bioconjugation Using N-Myristoyltransferase

    • Karin F. K. Ejendal, Julia G. Fraseur, Tamara L. Kinzer-Ursem
    Pages 149-165

  13. One-Step Fluorescent Protein Labeling by Tubulin Tyrosine Ligase

    • Dominik Schumacher, Heinrich Leonhardt, Christian P. R. Hackenberger, Jonas Helma
    Pages 167-189

  14. Enzymatically Catalyzed Radiofluorination of Biomolecules

    • Christopher R. Drake, Michael J. Evans, Henry F. VanBrocklin
    Pages 191-205

  15. Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase

    • Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, Mark D. Distefano
    Pages 207-219

  16. Homogeneous Antibody–Drug Conjugates via Glycoengineering

    • Feng Tang, Wei Shi, Wei Huang
    Pages 221-238

  17. Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselective Rapid Azo-Coupling Reaction

    • Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, Abhishek Chatterjee
    Pages 239-251

  18. Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria

    • Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, Andreas Plückthun
    Pages 253-273

  19. Site-Specific Photocrosslinking to Immunoglobulin G Using Photoreactive Antibody-Binding Domains

    • Fabiana Zappala, Andrew Tsourkas
    Pages 275-286

  20. SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

    • Xiao-Wei Wang, Wen-Bin Zhang
    Pages 287-300
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Keywords

About this book

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.

Editors and Affiliations

  • Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium

    Sam Massa

  • In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium

    Nick Devoogdt

Bibliographic Information

  • Book Title: Bioconjugation

  • Book Subtitle: Methods and Protocols

  • Editors: Sam Massa, Nick Devoogdt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9654-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9653-7Published: 23 July 2019

  • Softcover ISBN: 978-1-4939-9656-8Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9654-4Published: 22 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 317

  • Number of Illustrations: 37 b/w illustrations, 47 illustrations in colour

  • Topics: Antibodies, Protein Science, Pharmacology/Toxicology

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