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Immunoproteomics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kelly M. Fulton

    1. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Susan M. Twine

    1. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2024)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to the Immune System

    • Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, Felicity Stark
    Pages 1-24

  3. Immunoproteomics Methods and Techniques

    • Kelly M. Fulton, Isabel Baltat, Susan M. Twine
    Pages 25-58

  4. Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification

    • Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, Susan M. Twine
    Pages 59-78

  5. Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology

    • Greg Harris, Wangxue Chen
    Pages 79-94

  6. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

    • Timothy D. Veenstra
    Pages 95-102

  7. Enriching for Low-Abundance Serum Proteins Using ProteoMiner™ and Protein-Level HPLC

    • Sophie Moggridge, Kelly M. Fulton, Susan M. Twine
    Pages 103-117

  8. Identification of the Antigen Content of Electroimmunoprecipitates

    • N. Helena Beyer, Niels H. H. Heegaard
    Pages 119-135

  9. Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay

    • Christine M. Tin, Stanislav V. Sosnovtsev
    Pages 137-152

  10. Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum

    • Christie E. Delaney, John F. Kelly, Wen Ding, Arsalan S. Haqqani
    Pages 153-166

  11. Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Transfer

    • Tammy-Lynn Tremblay, Jennifer J. Hill
    Pages 167-180

  12. Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery

    • Elisa Beghetto, Nicola Gargano
    Pages 181-198

  13. Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray

    • Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall
    Pages 199-211

  14. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

    • Fei Wen, Mason R. Smith, Huimin Zhao
    Pages 213-234

  15. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification

    • Sarah A. Penny, Stacy A. Malaker
    Pages 235-243

  16. Enhanced Mass Spectrometry Detection of MHC Peptides

    • Rui Chen, Jianjun Li
    Pages 245-257

  17. Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles

    • Rui Chen, Jianjun Li
    Pages 259-268

  18. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways

    • Stacy A. Malaker, Michael J. Ferracane
    Pages 269-285

  19. Genome-Based Bioinformatic Prediction of Major Histocompatibility (MHC)

    • Simon J. Foote
    Pages 287-300

  20. Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach

    • Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, Shashi Gujar
    Pages 301-307
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Keywords

About this book

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field. 


Editors and Affiliations

  • Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, Canada

    Kelly M. Fulton, Susan M. Twine

Bibliographic Information

  • Book Title: Immunoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Kelly M. Fulton, Susan M. Twine

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9597-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9596-7Published: 31 July 2019

  • Softcover ISBN: 978-1-4939-9599-8Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9597-4Published: 30 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 380

  • Number of Illustrations: 18 b/w illustrations, 57 illustrations in colour

  • Topics: Immunology, Proteomics

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