Comparative Embryo Culture

1. Front Matter

   Pages i-xi

   PDF

2. Historical Note: Lessons Learned from Developing the First Semi-Defined Medium for Culture of One-Cell Rabbit Embryos to Expanding Blastocysts

   • Michael T. Kane

   Pages 1-12

3. Culture of the Mouse Preimplantation Embryo

   • David K. Gardner, Thi T. Truong

   Pages 13-32

4. In Vitro Culture of Rat Preimplantation Embryos

   • Yuksel Agca

   Pages 33-43

5. Enabling Hamster Embryo Culture System: Development of Preimplantation Embryos

   • Polani B. Seshagiri, Venkatappa Vani

   Pages 45-61

6. Culture of Preimplantation Rabbit Embryos

   • Michael T. Kane

   Pages 63-91

7. In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos

   • Bethany K. Redel, Lee D. Spate, Randall S. Prather

   Pages 93-103

8. In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Medium (PBM)

   • Tomomi Mito, Hiroyoshi Hoshi

   Pages 105-113

9. Production and Culture of the Bovine Embryo

   • Paula Tríbulo, Rocío Melissa Rivera, Martha Sofia Ortega Obando, Elizabeth Ann Jannaman, Peter J. Hansen

   Pages 115-129

10. In Vitro Embryo Production in Sheep

    • S. Mondal, A. Mor, I. J. Reddy, S. Nandi, P. S. P. Gupta, A. Mishra

    Pages 131-140

11. In Vitro Culture of Embryos from LOPU-Derived Goat Oocytes

    • Joanna Maria G. Souza-Fabjan, Ribrio Ivan T. P. Batista, Vicente José F. Freitas, Pascal Mermillod

    Pages 141-153

12. Small Ruminants: Prepubertal Oocyte Donors

    • Dolors Izquierdo, María-Gracia Catalá, María-Teresa Paramio

    Pages 155-163

13. In Vitro Production of Bison Embryos

    • Jennifer P. Barfield

    Pages 165-177

14. In Vitro Embryo Production in Buffalo Species (Bubalus bubalis)

    • Marcello Rubessa, Lucia Boccia, Serena Di Francesco

    Pages 179-190

15. In Vitro Culture of Deer Embryos

    • Debra K. Berg, Ampika Thongphakdee

    Pages 191-207

16. In Vitro Culture of Camelid Embryos

    • Islam M. Saadeldin, Ayman Abdel-Aziz Swelum, Abdullah N. Alowaimer

    Pages 209-218

17. In Vitro Culture of Embryos from Horses

    • Elaine M. Carnevale, Lisa J. Maclellan, Jo Anne E. Stokes

    Pages 219-227

18. In Vitro Culture of Embryos from Domestic Cats

    • Jason R. Herrick

    Pages 229-246

19. The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer

    • J. B. Nagashima, A. J. Travis, N. Songsasen

    Pages 247-267

20. Culture of Mink Preimplantation Embryos

    • Jane C. Fenelon, Bruce D. Murphy

    Pages 269-277

This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.  Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.

• Biomedical research
• Blastocysts
• Fertility
• Embryonic development
• Comparative studies
• Cell culture

• Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, USA

  Jason R. Herrick

Policies and ethics