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Neural Crest Cells

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1976)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

    • Kwaku Dad Abu-Bonsrah, Serena Viventi, Donald F. Newgreen, Mirella Dottori
    Pages 37-47
  3. Deciphering the Neural Crest Contribution to Cephalic Development with Avian Embryos

    • Moussab Alrajeh, Zuzana Vavrusova, Sophie E. Creuzet
    Pages 55-70
  4. Technologies for Live Imaging of Enteric Neural Crest-Derived Cells

    • Marlene M. Hao, Annette J. Bergner, Donald F. Newgreen, Hideki Enomoto, Heather M. Young
    Pages 97-105
  5. Mouse Embryo Culture for the Study of Neural Crest Cells

    • William A. Muñoz, Paul A. Trainor
    Pages 107-119
  6. Laser Capture Microdissection of Murine Embryonic Neural Crest Cells

    • Robert M. Greene, Irina Smolenkova, Michele Pisano
    Pages 121-133
  7. In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration

    • Elias H. Barriga, Adam Shellard, Roberto Mayor
    Pages 135-152
  8. Proteomics in Neural Crest Cell Research

    • Peter McCarthy, Quenten Schwarz
    Pages 153-165
  9. Neural Crest Methodologies in Zebrafish and Medaka

    • Kleio Petratou, Karen Camargo-Sosa, Ruqaiya Al Jabri, Yusuke Nagao, Robert Neil Kelsh
    Pages 167-184
  10. Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity

    • Reyna Deeya Ballim, Yusuke Nagao, Robert Neil Kelsh
    Pages 195-206
  11. Back Matter

    Pages 223-230

About this book

This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike.

Editors and Affiliations

  • Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, Australia

    Quenten Schwarz, Sophie Wiszniak

Bibliographic Information

  • Book Title: Neural Crest Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Quenten Schwarz, Sophie Wiszniak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9412-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9411-3Published: 12 April 2019

  • Softcover ISBN: 978-1-4939-9414-4Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9412-0Published: 11 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 230

  • Number of Illustrations: 36 illustrations in colour

  • Topics: Developmental Biology, Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access