Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1485)
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Table of contents (23 protocols)
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Front Matter
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Overview Chapters
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Front Matter
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Protocols Chapters
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Front Matter
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About this book
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
Keywords
Editors and Affiliations
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School of Biotechnology, Dublin City University, Dublin, Ireland
Dermot Walls
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Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland
Sinéad T. Loughran
Bibliographic Information
Book Title: Protein Chromatography
Book Subtitle: Methods and Protocols
Editors: Dermot Walls, Sinéad T. Loughran
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6412-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6410-9Published: 12 October 2016
Softcover ISBN: 978-1-4939-8191-5Published: 27 June 2018
eBook ISBN: 978-1-4939-6412-3Published: 11 October 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 426
Number of Illustrations: 19 b/w illustrations, 21 illustrations in colour
Topics: Protein Science