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Skeletal Muscle Regeneration in the Mouse

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Michael Kyba

    1. Lillehei Heart Institute, University of Minnesota, Minneapolis, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1460)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Injury Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle’s Capacity to Generate Force

      • Jarrod A. Call, Dawn A. Lowe
      Pages 3-18

    3. Volumetric Muscle Loss

      • Beth E. Pollot, Benjamin T. Corona
      Pages 19-31

    4. Freeze Injury of the Tibialis Anterior Muscle

      • Gengyun Le, Dawn A. Lowe, Michael Kyba
      Pages 33-41

    5. Synergist Ablation as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle

      • Tyler J. Kirby, John J. McCarthy, Charlotte A. Peterson, Christopher S. Fry
      Pages 43-52

    6. Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride

      • Matthew T. Tierney, Alessandra Sacco
      Pages 53-60

    7. Cardiotoxin Induced Injury and Skeletal Muscle Regeneration

      • Glynnis A. Garry, Marie Lue Antony, Daniel J. Garry
      Pages 61-71

    8. Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal Muscle

      • Patrizia Pessina, Pura Muñoz-Cánoves
      Pages 73-82

  3. Evaluating Progenitor Cells

    1. Front Matter

      Pages 83-83
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    2. Isolation, Cryosection and Immunostaining of Skeletal Muscle

      • Huascar P. Ortuste Quiroga, Katsumasa Goto, Peter S. Zammit
      Pages 85-100

    3. Isolation of Mouse Periocular Tissue for Histological and Immunostaining Analyses of the Extraocular Muscles and Their Satellite Cells

      • Pascal Stuelsatz, Zipora Yablonka-Reuveni
      Pages 101-127

    4. Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining

      • Mayank Verma, Bhavani SR Murkonda, Yoko Asakura, Atsushi Asakura
      Pages 129-140

    5. Isolation, Culture, Functional Assays, and Immunofluorescence of Myofiber-Associated Satellite Cells

      • Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, Bradley B. Olwin
      Pages 141-162

    6. Flow Cytometry and Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation

      • Robert W. Arpke, Michael Kyba
      Pages 163-179

    7. Noninvasive Tracking of Quiescent and Activated Muscle Stem Cell (MuSC) Engraftment Dynamics In Vivo

      • Andrew T. V. Ho, Helen M. Blau
      Pages 181-189

    8. Myogenic Progenitors from Mouse Pluripotent Stem Cells for Muscle Regeneration

      • Alessandro Magli, Tania Incitti, Rita C. R. Perlingeiro
      Pages 191-208

    9. Assaying Human Myogenic Progenitor Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient Mice

      • Maura H. Parker
      Pages 209-221

    10. Methods for Mitochondria and Mitophagy Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle

      • Laura García-Prat, Marta Martínez-Vicente, Pura Muñoz-Cánoves
      Pages 223-240

    11. Identification, Isolation, and Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle

      • Akiyoshi Uezumi, Takehiro Kasai, Kunihiro Tsuchida
      Pages 241-253
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Keywords

About this book

This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.

Editors and Affiliations

  • Lillehei Heart Institute, University of Minnesota, Minneapolis, USA

    Michael Kyba

About the editor

Michael Kyba, Ph.D. Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE Minneapolis, MN 55455, USA

Bibliographic Information

  • Book Title: Skeletal Muscle Regeneration in the Mouse

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Kyba

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3810-0

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3808-7Published: 06 August 2016

  • Softcover ISBN: 978-1-4939-8145-8Published: 07 June 2018

  • eBook ISBN: 978-1-4939-3810-0Published: 05 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 346

  • Number of Illustrations: 10 b/w illustrations, 87 illustrations in colour

  • Topics: Molecular Medicine

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