Skip to main content
  • Book
  • © 2016

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1448)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Recent Improvements in LV Construction, Production, and Transduction

    1. Front Matter

      Pages 1-1
    2. Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown

      • Angeline de Bruyns, Ben Geiling, David Dankort
      Pages 3-21
    3. Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G

      • NataÅ¡a Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt, Christian Thirion
      Pages 49-61
  3. New LV Targets and Applications

    1. Front Matter

      Pages 63-63
    2. Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors

      • Maike Stahlhut, Axel Schambach, Olga S. Kustikova
      Pages 65-76
    3. Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer

      • Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, Robert Winston
      Pages 95-106
    4. The LAM-PCR Method to Sequence LV Integration Sites

      • Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, Manfred Schmidt
      Pages 107-120
    5. Conditional RNAi Using the Lentiviral GLTR System

      • Elisabeth Pfeiffenberger, Reinhard Sigl, Stephan Geley
      Pages 121-138
    6. Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies

      • Aurélien Lathuilière, Bernard L. Schneider
      Pages 139-155
  4. Integrase-Mutant Lvs

    1. Front Matter

      Pages 157-157
    2. Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector-Transduced 293T Cell Line

      • Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, M. K. Azaham A. Hamid
      Pages 159-173
    3. Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

      • Ngoc B. Lu-Nguyen, Martin Broadstock, Rafael J. Yáñez-Muñoz
      Pages 175-184
    4. Development of Lentiviral Vectors for Targeted Integration and Protein Delivery

      • Diana Schenkwein, Seppo Ylä-Herttuala
      Pages 185-198
  5. Production, Detection, and Engineering of Exosomes

    1. Front Matter

      Pages 199-199
    2. Biogenesis and Functions of Exosomes and Extracellular Vesicles

      • Florian Dreyer, Andreas Baur
      Pages 201-216

About this book

This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles.  Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Istituto Superiore di Sanità, National AIDS Center, Rome, Italy

    Maurizio Federico

Bibliographic Information

  • Book Title: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

  • Editors: Maurizio Federico

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3753-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3751-6Published: 18 June 2016

  • Softcover ISBN: 978-1-4939-8129-8Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3753-0Published: 17 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 276

  • Number of Illustrations: 14 b/w illustrations, 33 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access