Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Neuromethods (NM, volume 99)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (30 protocols)
-
Front Matter
-
FFPE Sections
-
Front Matter
-
-
Model Organisms
-
Front Matter
-
-
Systematic Gene Expression Analysis
-
Front Matter
-
-
Evo-Devo
-
Front Matter
-
About this book
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.
Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
Editors and Affiliations
-
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
Giselbert Hauptmann
Bibliographic Information
Book Title: In Situ Hybridization Methods
Editors: Giselbert Hauptmann
Series Title: Neuromethods
DOI: https://doi.org/10.1007/978-1-4939-2303-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2302-1Published: 19 February 2015
Softcover ISBN: 978-1-4939-4463-7Published: 05 October 2016
eBook ISBN: 978-1-4939-2303-8Published: 18 February 2015
Series ISSN: 0893-2336
Series E-ISSN: 1940-6045
Edition Number: 1
Number of Pages: XX, 592
Number of Illustrations: 15 b/w illustrations, 94 illustrations in colour
Topics: Neurosciences, Neurobiology