Editors:
- Includes cutting-edge methods and protocols involving insoluble proteins
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1258)
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Table of contents (24 protocols)
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Front Matter
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Recombinant Protein Production in Escherichia coli
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Strategies to Produce Insoluble Proteins in Cell-Free Expression Systems
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Recombinant Protein Production in Lactic Acid Bacteria (LAB)
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Front Matter
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Recombinant Protein Production in Yeast
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Front Matter
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Recombinant Protein Production in Insect Cells-Baculovirus
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Front Matter
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Recombinant Protein Production in Mammalian Cells
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Recombinant Protein Production in Other Systems
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Front Matter
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About this book
With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.
Editors and Affiliations
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Departament de Genètica i de Microbiologia, CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) and Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain
Elena García-Fruitós
Bibliographic Information
Book Title: Insoluble Proteins
Book Subtitle: Methods and Protocols
Editors: Elena García-Fruitós
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2205-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2204-8Published: 02 December 2014
Softcover ISBN: 978-1-4939-4675-4Published: 23 August 2016
eBook ISBN: 978-1-4939-2205-5Published: 01 December 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 425
Number of Illustrations: 31 b/w illustrations, 41 illustrations in colour
Topics: Protein Science