Ovarian Cancer

1. Front Matter

   Pages i-xiv

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2. Clinical Perspectives

   1. Front Matter

      Pages 1-1

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   2. Clinical Staging of Ovarian Cancer

      • Andrea S. O’Shea

      Pages 3-10

   3. Pathologic Classification of Ovarian Cancer

      • Stephanie M. McGregor

      Pages 11-40

3. Tumor Microenvironment

   1. Front Matter

      Pages 41-41

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   2. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer

      • Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, Manish S. Patankar

      Pages 43-58

   3. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors

      • Veronica D. Gonzalez, Ying-Wen Huang, Wendy J. Fantl

      Pages 59-94

   4. Processing and Analysis of Ascites

      • Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, Pamela K. Kreeger

      Pages 95-104

   5. Multispectral Staining and Analysis of Extracellular Matrix

      • Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, Kristyn S. Masters

      Pages 105-119

   6. Quantitative Analysis of the Extracellular Matrix by Immunoblot

      • Kaitlin C. Fogg

      Pages 121-132

4. Cell Isolation

   1. Front Matter

      Pages 133-133

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   2. Multiparameter Single-Cell Characterization of Ovarian Intratumor Heterogeneity

      • Kristin G. Beaumont, Christina Andreou, Ethan Ellis, Robert Sebra

      Pages 135-146

   3. Culturing Primary Human Mesothelial Cells

      • Mary Mullen, Hollie Noia, Katherine Fuh

      Pages 147-154

   4. Isolation of Normal and Cancer-Associated Fibroblasts

      • Katarzyna Zawieracz, Mark A. Eckert

      Pages 155-165

   5. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum

      • Abir Mukherjee

      Pages 167-175

5. Model Systems

   1. Front Matter

      Pages 177-177

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   2. Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF)

      • Angela Russo, Joanna E. Burdette

      Pages 179-187

   3. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer

      • Matthew Dean

      Pages 189-198

   4. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer

      • Elizabeth I. Harper, Tyvette S. Hilliard

      Pages 199-216

   5. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance : 3D Co-culture Methods

      • Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, Geeta Mehta

      Pages 217-245

This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches.

• Tumor microenvironment
• Cell isolation
• Ovary
• Fallopian tube
• Cancer cell lines
• Tumor development
• Therapy targets

• Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, USA

  Pamela K. Kreeger

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