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Immunohistochemistry and Immunocytochemistry

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Luis Del Valle

    1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2422)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art

    • Luis Del Valle
    Pages 1-16

  3. Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present

    • Carlos Ortiz Hidalgo
    Pages 17-31

  4. Sample Preparation and Fixation for Histology and Pathology

    • Enrique Agustín Sampedro-Carrillo
    Pages 33-45

  5. Tissue Processing

    • Sarah J. Aziz, Cady E. Zeman-Pocrnich
    Pages 47-63

  6. Antigen Retrieval and Signal Amplification

    • Logan Dunkenberger, Luis Del Valle
    Pages 65-74

  7. Primary Antibody Selection and Blocking Techniques for Immunohistochemistry

    • Lori A. Peton, Miguel Reyes-Múgica
    Pages 75-84

  8. Probes for Fluorescent Visualization of Specific Cellular Organelles

    • Timothy Paul Foster
    Pages 85-124

  9. Enzymatic Immunohistochemistry

    • Rodolfo Henrich Lobo
    Pages 125-129

  10. Fluorescent Immunohistochemistry

    • Vanessa Moreno, Elizabeth A. Smith, Sergio Piña-Oviedo
    Pages 131-146

  11. Double Labeling Fluorescent Immunocytochemistry

    • Monika Rak, Krzysztof Reiss
    Pages 147-161

  12. Multiplexing and Spectral Microscopy

    • Logan Dunkenberger, Adriana Zapata, Luis Del Valle
    Pages 163-177

  13. Fluorescent In Situ Hybridization (FISH)

    • Jeanette M. Ramos
    Pages 179-189

  14. Proximity Ligation Assay (PLA)

    • Muhammad S. Alam
    Pages 191-201

  15. Immunohistochemistry, Quality Control, and Principles of Validation in the Central Nervous System

    • Verónica Velasco-Vales, Danny Soria-Céspedes, Teresa Cristina Cuesta-Mejías, Norma Cecilia Padrón-Pérez
    Pages 203-216

  16. Culture and Phenotyping of Glial Cell Cultures, Gliospheres , and Neurospheres

    • Luis Del Valle, Krzysztof Reiss, Amanda Parker Struckhoff
    Pages 217-232

  17. ER+ Breast Cancer Mammosphere Formation and Analysis

    • Ciera S. Singleton, Leo Li-Ying Chan, Kelsey J. McCulley, Sarah L. Kessel, Luis Del Valle, Judy S. Crabtree
    Pages 233-245

  18. Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSC) by Flow Cytometry

    • Maria Dulfary Sanchez-Pino
    Pages 247-261

  19. Comet Assay for the Detection of Single and Double-Strand DNA Breaks

    • Logan Dunkenberger, Krzysztof Reiss, Luis Del Valle
    Pages 263-269

  20. Fluorescent-Activated Cell Sorting ( Flow Cytometry )

    • Luis F. Carrillo
    Pages 271-281
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Keywords

About this book

This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field

Editors and Affiliations

  • Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, USA

    Luis Del Valle

Bibliographic Information

  • Book Title: Immunohistochemistry and Immunocytochemistry

  • Book Subtitle: Methods and Protocols

  • Editors: Luis Del Valle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1948-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1947-6Published: 04 December 2021

  • Softcover ISBN: 978-1-0716-1950-6Published: 05 December 2022

  • eBook ISBN: 978-1-0716-1948-3Published: 03 December 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 286

  • Number of Illustrations: 7 b/w illustrations, 43 illustrations in colour

  • Topics: Immunology

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