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Estrogen Receptors

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2418)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Antibody Validation for Estrogen Receptor Beta

    • Madeleine Birgersson, Borbala Katona, Cecilia Lindskog, Fredrik Pontén, Cecilia Williams
    Pages 1-23
  3. Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 /GPER1 Activation

    • Sara Clark, Kevin Pollard, Jennifer Rainville, Nandini Vasudevan
    Pages 25-39
  4. Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain

    • Ana C. Ribeiro, Mihaela A. Stavarache, Sergei Musatov, Donald W. Pfaff
    Pages 53-61
  5. Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils

    • Sawako Shindo, Rick Moore, MyeongJin Yi, Masahiko Negishi
    Pages 63-75
  6. Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen

    • Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe, Eugene D. Albrecht
    Pages 77-93
  7. Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR

    • Michelle L. Booze, Kathleen M. Eyster
    Pages 113-127
  8. PET Imaging of Estrogen Receptors Using 18F-Based Radioligands

    • Manoj Kumar, Kelley Salem, Justin J. Jeffery, Amy M. Fowler
    Pages 129-151
  9. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model

    • Daniel A. Gorelick, Caroline Lucia, Ruixin Hao, Silvia Karim, Maria Bondesson
    Pages 173-185
  10. Assessing Estrogenic Activity of Classical Estrogen Receptor -Binding Compounds

    • Richard A. Pepermans, Eric R. Prossnitz
    Pages 187-201
  11. An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta

    • Rajitha Indukuri, Anastasios Damdimopoulos, Cecilia Williams
    Pages 203-221
  12. Polysome Fractionation for Transcriptome-Wide Studies of mRNA Translation

    • Johannes Ristau, Kathleen Watt, Christian Oertlin, Ola Larsson
    Pages 223-241
  13. Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation

    • Christian Oertlin, Kathleen Watt, Johannes Ristau, Ola Larsson
    Pages 243-268
  14. Whole-Genome Genotyping Using DNA Microarrays for Population Genetics

    • Austin J. Van Asselt, Erik A. Ehli
    Pages 269-287
  15. Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells

    • Amena Archer, Claudia Kutter, Cecilia Williams
    Pages 313-343

About this book

This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative andpractical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.

Editors and Affiliations

  • Sanford School of Medicine, University of South Dakota, Vermillion, USA

    Kathleen M. Eyster

Bibliographic Information

  • Book Title: Estrogen Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Kathleen M. Eyster

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1920-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1919-3Published: 05 February 2022

  • Softcover ISBN: 978-1-0716-1922-3Published: 05 February 2023

  • eBook ISBN: 978-1-0716-1920-9Published: 04 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 427

  • Number of Illustrations: 13 b/w illustrations, 68 illustrations in colour

  • Topics: Physiology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access