Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2416)
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Table of contents (17 protocols)
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Front Matter
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Back Matter
Keywords
About this book
This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.
Editors and Affiliations
Bibliographic Information
Book Title: Human Naïve Pluripotent Stem Cells
Editors: Peter Rugg-Gunn
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1908-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1907-0Published: 07 December 2021
Softcover ISBN: 978-1-0716-1910-0Published: 08 December 2022
eBook ISBN: 978-1-0716-1908-7Published: 06 December 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 288
Number of Illustrations: 66 b/w illustrations
Topics: Stem Cells, Regenerative Medicine/Tissue Engineering, Cell Biology