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Biomedical Engineering Technologies

Volume 2

  • I?ncludes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2394)

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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xxiii
  2. Molecular and Cellular Analysis and Manipulation

    1. Front Matter

      Pages 1-1
    2. Development of a Multi-target Protein Biomarker Assay for Circulating Tumor Cells

      • Diya Li, Ceming Wang, Yingjia Ni, Yaoping Liu, Wei Wang, Siyuan Zhang et al.
      Pages 3-18
    3. Method to Isolate Dormant Cancer Cells from Heterogeneous Populations

      • Julian A. Preciado, Alptekin Aksan
      Pages 19-29
    4. Label-Free Morphological Phenotyping of In Vitro 3D Microtumors

      • Zoe Moscato, Devina Jaiswal, Krishna Dixit, Cooper J. Langanis, Kevin P. Claffey, Kazunori Hoshino
      Pages 31-46
    5. High-Throughput Microenvironment Microarray (MEMA) High-Resolution Imaging

      • Tiina A. Jokela, Michael E. Todhunter, Mark A. LaBarge
      Pages 47-64
    6. Real-Time Analysis of AKT Signaling Activities at Single-Cell Resolution Using Cyclic Peptide-Based Probes

      • Fei Ji, Siwen Wang, Shiqun Shao, Priyanka Sarkar, Min Xue
      Pages 65-80
    7. Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine

      • Donald J. Johann Jr., Sarah Laun, Ik Jae Shin, Robert Weigman, Owen Stephens, Adam Roberge et al.
      Pages 93-107
    8. Functionalized Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations

      • Andrea Gardner, Daylin Morgan, Aziz Al’Khafaji, Amy Brock
      Pages 109-131
    9. Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors

      • Anna Bianchi-Smiraglia, Mikhail A. Nikiforov
      Pages 163-169
    10. Node-Pore Sensing for Characterizing Cells and Extracellular Vesicles

      • Thomas Carey, Brian Li, Lydia L. Sohn
      Pages 171-183
    11. Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobes

      • Yuan Wan, Mackenzie Maurer, Si-Yang Zheng
      Pages 185-197
    12. Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR

      • Alejandro Stark, Alexander Trick, Thomas R. Pisanic II, Tza-Huei Wang
      Pages 199-209
    13. Molecular Imaging of HER2 in Patient Tissues with Touch Prep-Quantitative Single Molecule Localization Microscopy

      • Devin L. Wakefield, Steven J. Tobin, Daniel Schmolze, Tijana Jovanovic-Talisman
      Pages 231-248
    14. Microchip Free-Flow Electrophoresis for Bioanalysis, Sensing, and Purification

      • William E. Arter, Kadi L. Saar, Therese W. Herling, Tuomas P. J. Knowles
      Pages 249-266
    15. Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses

      • Andrey P. Tikunov, Jeremiah D. Tipton, Timothy J. Garrett, Sachi V. Shinde, Hong Jin Kim, David A. Gerber et al.
      Pages 267-298

About this book

This volume provides detailed technical protocols on current biomedical technologies and examples of their applications and capabilities. Chapters focus on molecular and cellular analytical methods, experimental new drug delivery approaches, guided surgery, implants and tissue engineering.     Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and practical, Biomedical Engineering Technologies, Volume 2 provides technical details in descriptions of major technologies by experts in the field.

Editors and Affiliations

  • Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, USA

    Avraham Rasooly, Houston Baker

  • Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, USA

    Miguel R. Ossandon

Bibliographic Information

  • Book Title: Biomedical Engineering Technologies

  • Book Subtitle: Volume 2

  • Editors: Avraham Rasooly, Houston Baker, Miguel R. Ossandon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1811-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1810-3Published: 31 January 2022

  • Softcover ISBN: 978-1-0716-1813-4Published: 01 February 2023

  • eBook ISBN: 978-1-0716-1811-0Published: 30 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXIII, 910

  • Number of Illustrations: 49 b/w illustrations, 241 illustrations in colour

  • Topics: Biotechnology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access