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Oxytocin

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Eryn L. Werry

    1. School of Chemistry, The University of Sydney, Camperdown, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Tristan A. Reekie

    1. Research School of Chemistry, Australian National University, Canberra, Australia

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    You can also search for this editor in PubMed   Google Scholar

  3. Michael Kassiou

    1. School of Chemistry, The University of Sydney, Camperdown, Australia

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2384)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Importance of Experimental Investigation of the Peripheral Oxytocin System

    • Stephen J. Assinder
    Pages 1-27

  3. Human Myometrial Contractility Assays

    • Sarah Arrowsmith
    Pages 29-42

  4. The Effect of Oxytocin on Intracellular Ca2+ Release in Cardiac Cells

    • Anatoliy Shmygol, Sanaa H. S. H. Alameri, Khadeijah Y. K. M. Almemari, Naema M. A. J. Alsuwaidi, Ifrah I. Ali
    Pages 43-52

  5. The Importance of Experimental Investigation of the CNS Oxytocin System

    • Kelsie A. Boulton, Adam J. Guastella
    Pages 53-65

  6. The Role of Oxytocin in Social Circuits and Social Behavior in Dementia

    • Olivier Piguet, Rebekah M. Ahmed, Fiona Kumfor
    Pages 67-80

  7. Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region

    • Leonora King, Stephanie Robins, Gary Chen, Gustavo Turecki, Phyllis Zelkowitz
    Pages 81-103

  8. Using Receptor Autoradiography to Visualize and Quantify Oxytocin and Vasopressin 1a Receptors in the Human and Nonhuman Primate Brain

    • Sara M. Freeman
    Pages 105-125

  9. Linking Central Gene Expression Patterns and Mental States Using Transcriptomics and Large-Scale Meta-Analysis of fMRI Data: A Tutorial and Example Using the Oxytocin Signaling Pathway

    • Jaroslav Rokicki, Daniel S. Quintana, Lars T. Westlye
    Pages 127-137

  10. CRISPR/Cas9-Mediated Genetic Engineering to Generate a Disease Model Prairie Vole , Based on Species-Optimized Assisted Reproductive Technology

    • Kengo Horie, Katsuhiko Nishimori
    Pages 139-152

  11. The Current Status of Drug Discovery for the Oxytocin Receptor

    • Philippe E. Nashar, Aidan A. Whitfield, Jiri Mikusek, Tristan A. Reekie
    Pages 153-174

  12. Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues

    • Thomas Kremsmayr, Markus Muttenthaler
    Pages 175-199

  13. Employing the Operational Model to Measure System-Independent OTR Efficacy

    • Kiyan Afzali, Mark Connor
    Pages 201-220

  14. Use of BRET to Measure β-Arrestin Recruitment at Oxytocin and Vasopressin Receptors

    • Edin Muratspahić, Jasmin Gattringer, Christian W. Gruber
    Pages 221-229

  15. Measuring Affinity of Ligands to the Oxytocin Receptor Using Radioligand Binding

    • Eryn L. Werry, Michael Kassiou
    Pages 231-245

  16. Use of Cocultures to Measure the Blood–Brain Barrier Permeability of Oxytocin

    • Yasuhiko Yamamoto, Ai Harashima, Sei-ichi Munesue, Haruhiro Higashida
    Pages 247-255

  17. Intracerebral Delivery of an OTR Antagonist and Subsequent Behavioral Testing in Prairie Voles

    • Yu Hirota, Chisato Otsuki, Erika Kamura, Shinichi Mitsui
    Pages 257-269

  18. Back Matter

    Pages 271-272
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Keywords

About this book

This detailed book explores techniques for further elucidating the peripheral and central roles of oxytocin as well as techniques key to oxytocin receptor-related drug discovery. The first set of chapters explore this neuropeptide’s peripheral and central effects, such as regulation of myometrial contraction, induction of cardioprotective effects, and the facilitation of pro-social behaviors. The book then continues by delving into a comprehensive pharmacological characterization of oxytocin receptor ligands and ligands of other key receptors such as the vasopressin receptor family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oxytocin: Methods and Protocols is an ideal guide for researchers seeking to further our knowledge of the varied and power effects of oxytocin within the central nervous system.

Editors and Affiliations

  • School of Chemistry, The University of Sydney, Camperdown, Australia

    Eryn L. Werry, Michael Kassiou

  • Research School of Chemistry, Australian National University, Canberra, Australia

    Tristan A. Reekie

Bibliographic Information

  • Book Title: Oxytocin

  • Book Subtitle: Methods and Protocols

  • Editors: Eryn L. Werry, Tristan A. Reekie, Michael Kassiou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1759-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1758-8Published: 23 September 2021

  • Softcover ISBN: 978-1-0716-1761-8Published: 25 September 2021

  • eBook ISBN: 978-1-0716-1759-5Published: 22 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 272

  • Number of Illustrations: 9 b/w illustrations, 38 illustrations in colour

  • Topics: Neurochemistry, Neurosciences, Pharmacology/Toxicology

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