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Therapeutic Antibodies

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Gunnar Houen

    1. Department of Neurology, Rigshospitalet, Glostrup, Denmark

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2313)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Therapeutic Antibodies : An Overview

    • Gunnar Houen
    Pages 1-25

  3. Treatment of Autoimmune Diseases with Therapeutic Antibodies : Lessons Learned from PID Patients Allow for Stratification of the Infection Risk

    • Joyce J. B. C. van Beers, Jan G. M. C. Damoiseaux
    Pages 27-44

  4. B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases

    • Jan G. M. C. Damoiseaux, Joyce J. B. C. van Beers, Matthias Busch, Pieter van Paassen
    Pages 45-55

  5. Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods

    • Adriana-Michelle Wolf Pérez, Nikolai Lorenzen, Michele Vendruscolo, Pietro Sormanni
    Pages 57-113

  6. The Therapeutic Antibody Profiler for Computational Developability Assessment

    • Matthew I. J. Raybould, Charlotte M. Deane
    Pages 115-125

  7. Short Read-Length Next Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs

    • Landon Pastushok, Kris Barreto, C. Ronald Geyer
    Pages 127-141

  8. Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection

    • Xiaotian Zhong, Ashley Schwab, Weijun Ma, Caryl L. Meade, Jing Zhou, Aaron M. D’Antona et al.
    Pages 143-150

  9. Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris

    • Laia Montoliu-Gaya, Sandra Villegas
    Pages 151-167

  10. Purification of Therapeutic Antibodies by Protein A Affinity Chromatography

    • Anurag S. Rathore, Sharad Narnaware
    Pages 169-177

  11. Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies

    • Anurag S. Rathore, Vishwanath Hebbi
    Pages 179-186

  12. Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies

    • Noriko Iwamoto, Takashi Shimada
    Pages 187-205

  13. Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality

    • Detlev Suckau, Waltraud Evers, Eckhard Belau, Stuart Pengelley, Anja Resemann, Wilfred Tang et al.
    Pages 207-217

  14. Nano-Microscopy of Therapeutic Antibody Aggregates in Solution

    • Yukako Senga, Toshihiko Ogura, Hiroshi Imamura, Shinya Honda
    Pages 219-239

  15. Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering

    • Fabian Dingfelder, Anette Henriksen, Per-Olof Wahlund, Paolo Arosio, Nikolai Lorenzen
    Pages 241-258

  16. Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification

    • Lisa A. Vasicek, Daniel S. Spellman, Kevin P. Bateman
    Pages 259-268

  17. Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS

    • Feng Yang, Delia Li, Donald E. Walker
    Pages 269-279

  18. Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies

    • Nohemí Salinas-Jazmín, Emilio Medina-Rivero, Marco Antonio Velasco-Velázquez
    Pages 281-294

  19. Methods for Functional Characterization of FcRn Interactions with Therapeutic Antibodies and Fc-Fusion Proteins

    • Shan Chung, Yuwen L. Lin, Van Nguyen, Chang Liu
    Pages 295-303

  20. Method for Measurement of Antibody-Dependent Cellular Phagocytosis

    • Lynn Kamen, Benjamin Ordonia, Srividya Myneni, Shan Chung
    Pages 305-312
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Keywords

About this book

This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Therapeutic Antibodies: Methods and Protocols serves as an ideal guide for researchers working with the production of, research on, and development of therapeutic antibodies as well as for clinicians using therapeutic antibodies in daily work with patients.

Editors and Affiliations

  • Department of Neurology, Rigshospitalet, Glostrup, Denmark

    Gunnar Houen

Bibliographic Information

  • Book Title: Therapeutic Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Gunnar Houen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1450-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1449-5Published: 04 September 2021

  • Softcover ISBN: 978-1-0716-1452-5Published: 05 September 2022

  • eBook ISBN: 978-1-0716-1450-1Published: 03 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 347

  • Number of Illustrations: 10 b/w illustrations, 61 illustrations in colour

  • Topics: Biotechnology, Biological Techniques, Pharmacology/Toxicology

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