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Rice Genome Engineering and Gene Editing

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Anindya Bandyopadhyay

    1. Synthetic Biology, Biofuel and Genome Editing R&D, Reliance Industries Ltd, Navi Mumbai, India

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    You can also search for this editor in PubMed   Google Scholar

  2. Roger Thilmony

    1. Crop Improvement and Genetics Research Unit, USDA-ARS, Albany, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2238)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
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  2. Genetic Engineering and Tissue Culture of Rice

    1. Front Matter

      Pages 1-1
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    2. Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY)

      • Leyla T. Hathwaik, James G. Thomson, Roger Thilmony
      Pages 3-17

    3. TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology

      • Qinlong Zhu, Yao-Guang Liu
      Pages 19-35

    4. Morphogenic Regulators and Their Application in Improving Plant Transformation

      • Samson Nalapalli, Meral Tunc-Ozdemir, Yuejin Sun, Sivamani Elumalai, Qiudeng Que
      Pages 37-61

    5. A Rapid Method for Stably Transforming Rice Seeds

      • Sudheer Kumar, Neeti Sanan-Mishra
      Pages 63-68

    6. Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector

      • Shuvobrata Majumder, Karabi Datta, Swapan Kumar Datta
      Pages 69-79

    7. Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivars

      • Tanushri Kaul, Sonia Khan Sony, Nitya Meenakshi Raman, Khaled Fathy Abdel Motelb, Jyotsna Bharti
      Pages 81-91

  3. Genome Editing and Targeted Integration in Rice

    1. Front Matter

      Pages 93-93
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    2. Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts

      • Simon Sretenovic, Changtian Pan, Xu Tang, Yong Zhang, Yiping Qi
      Pages 95-113

    3. Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement

      • Amit Das, Pallavi Ghana, Bhojaraja Rudrappa, Rita Gandhi, Venkata Sresty Tavva, Amitabh Mohanty
      Pages 115-134

    4. Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

      • My Vo Thi Tra, Xiaojia Yin, Ishita Bajal, Christian Paolo Balahadia, William Paul Quick, Anindya Bandyopadhyay
      Pages 135-143

    5. Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing

      • Guanqing Liu, Yiping Qi, Tao Zhang
      Pages 145-172

    6. Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct

      • Martine Bes, Leo Herbert, Thibault Mounier, Anne-Cécile Meunier, Franz Durandet, Emmanuel Guiderdoni et al.
      Pages 173-191

    7. Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice

      • Xu Tang, Yiping Qi, Yong Zhang
      Pages 193-204

    8. Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9

      • Yesuf Teslim Yimam, Jianping Zhou, Sayed Abdul Akher, Xuelian Zheng, Yiping Qi, Yong Zhang
      Pages 205-219

    9. Rice Haploid Inducer Development by Genome Editing

      • Juntao Liu, Dawei Liang, Li Yao, Ya Zhang, Chunxia Liu, Yubo Liu et al.
      Pages 221-230

    10. FLP-Mediated Site-Specific Gene Integration in Rice

      • Vibha Srivastava
      Pages 231-240

    11. Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy

      • Rie Terada, Zenpei Shimatani
      Pages 241-257

  4. Bioinformatics, Cellular, and Molecular Analysis of Rice

    1. Front Matter

      Pages 259-259
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Keywords

About this book

This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.

Editors and Affiliations

  • Synthetic Biology, Biofuel and Genome Editing R&D, Reliance Industries Ltd, Navi Mumbai, India

    Anindya Bandyopadhyay

  • Crop Improvement and Genetics Research Unit, USDA-ARS, Albany, USA

    Roger Thilmony

Bibliographic Information

  • Book Title: Rice Genome Engineering and Gene Editing

  • Book Subtitle: Methods and Protocols

  • Editors: Anindya Bandyopadhyay, Roger Thilmony

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1068-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1067-1Published: 21 January 2021

  • Softcover ISBN: 978-1-0716-1070-1Published: 21 January 2022

  • eBook ISBN: 978-1-0716-1068-8Published: 20 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 378

  • Number of Illustrations: 11 b/w illustrations, 70 illustrations in colour

  • Topics: Plant Sciences, Food Microbiology, Genetics and Genomics

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