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cryoEM

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Tamir Gonen

    1. Howard Hughes Medical Institute Departments of Biological Chemistry and Physiology, University of California, Los Angeles, Los Angeles, USA

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  2. Brent L. Nannenga

    1. Chemical Engineering, School for Engineering of Matter, Transport and Energy; Center for Applied Structural Discovery Biodesign Institute, Arizona State University, Tempe, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2215)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Electron Cryotomography

    1. Front Matter

      Pages 1-1
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    2. Getting Started with In Situ Cryo-Electron Tomography

      • Daniel Serwas, Karen M. Davies
      Pages 3-23

    3. Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons

      • Ryan K. Hylton, Victoria H. Seader, Matthew T. Swulius
      Pages 25-48

    4. Practical Approaches for Cryo-FIB Milling and Applications for Cellular Cryo-Electron Tomography

      • Vinson Lam, Elizabeth Villa
      Pages 49-82

    5. In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography

      • Mohammed Kaplan, William J. Nicolas, Wei Zhao, Stephen D. Carter, Lauren Ann Metskas, Georges Chreifi et al.
      Pages 83-111

  3. Single Particle Analysis and Electron Cryomicroscopy

    1. Front Matter

      Pages 113-113
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    2. Progress Towards CryoEM: Negative-Stain Procedures for Biological Samples

      • Shane Gonen
      Pages 115-123

    3. Setting Up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection

      • Mark A. Herzik Jr
      Pages 125-144

    4. Multi-body Refinement of Cryo-EM Images in RELION

      • Takanori Nakane, Sjors H. W. Scheres
      Pages 145-160

    5. Evaluating Local and Directional Resolution of Cryo-EM Density Maps

      • Sriram Aiyer, Cheng Zhang, Philp R. Baldwin, Dmitry Lyumkis
      Pages 161-187

    6. Automated Modeling and Validation of Protein Complexes in Cryo-EM Maps

      • Tristan Cragnolini, Aaron Sweeney, Maya Topf
      Pages 189-223

  4. Electron Crystallography of 2D Crystals

    1. Front Matter

      Pages 225-225
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    2. 2D Electron Crystallography of Membrane Protein Single-, Double-, and Multi-Layered Ordered Arrays

      • Matthew C. Johnson, Yusuf M. Uddin, Kasahun Neselu, Ingeborg Schmidt-Krey
      Pages 227-245

    3. Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid–Protein Interaction

      • Ka-Yi Chan, Chloe Du Truong, Yu-Ping Poh, Po-Lin Chiu
      Pages 247-265

    4. Single Particle Analysis for High-Resolution 2D Electron Crystallography

      • Ricardo Righetto, Henning Stahlberg
      Pages 267-284

  5. Microcrystal Electron Diffraction (MicroED)

    1. Front Matter

      Pages 285-285
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    2. MicroED Sample Preparation and Data Collection For Protein Crystals

      • Guanhong Bu, Brent L. Nannenga
      Pages 287-297

    3. Detection of Microcrystals for CryoEM

      • Simon Weiss, Sandra Vergara, Guowu Lin, Guillermo Calero
      Pages 299-307

    4. Low-Dose Data Collection and Radiation Damage in MicroED

      • Johan Hattne
      Pages 309-319
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Keywords

About this book

This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology. 



Editors and Affiliations

  • Howard Hughes Medical Institute Departments of Biological Chemistry and Physiology, University of California, Los Angeles, Los Angeles, USA

    Tamir Gonen

  • Chemical Engineering, School for Engineering of Matter, Transport and Energy; Center for Applied Structural Discovery Biodesign Institute, Arizona State University, Tempe, USA

    Brent L. Nannenga

Bibliographic Information

  • Book Title: cryoEM

  • Book Subtitle: Methods and Protocols

  • Editors: Tamir Gonen, Brent L. Nannenga

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0966-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0965-1Published: 27 December 2020

  • Softcover ISBN: 978-1-0716-0968-2Published: 28 December 2021

  • eBook ISBN: 978-1-0716-0966-8Published: 26 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 350

  • Number of Illustrations: 15 b/w illustrations, 74 illustrations in colour

  • Topics: Biotechnology

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