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Plant Chemical Genomics

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Glenn R. Hicks

    1. Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Chunhua Zhang

    1. Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University, West Lafayette, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2213)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
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  2. Screening for Novel Small Molecules

    1. Front Matter

      Pages 1-1
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    2. Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated Signaling Events in Plants

      • Bibi Rafeiza Khan, Kent D. Chapman, Elison B. Blancaflor
      Pages 3-16

    3. Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitors

      • Emiko Okubo-Kurihara, Wen-Dee Ong, Yukio Kurihara, Natsumaro Kutsuna, Minami Matsui
      Pages 17-27

    4. Whole-Seedling-Based Chemical Genetic Screens in Arabidopsis

      • Shuai Huang, Xin Li
      Pages 29-37

    5. Identification of Type III Secretion Inhibitors for Plant Disease Management

      • Roger de Pedro Jové, Pau Sebastià, Marc Valls
      Pages 39-48

    6. Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture

      • Gian Pietro Di Sansebastiano
      Pages 49-58

  3. New Approaches to Variation: Peptide Screen for Novel Effectors

    1. Front Matter

      Pages 59-59
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    2. In Vivo Chemical Genomics with Random Cyclized Peptides

      • Tautvydas Shuipys, Maureen Clancy, Elizabeth Estrada-Johnson, Kevin M. Folta
      Pages 61-70

    3. Interfering Peptides Targeting Protein–Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence

      • Alexander Hofmann, Alexander Minges, Georg Groth
      Pages 71-85

  4. Chemical Genomics in Hormone Signaling

    1. Front Matter

      Pages 87-87
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    2. The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling

      • Kjell De Vriese, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, Steffen Vanneste
      Pages 89-98

    3. Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening

      • Jorge Lozano-Juste, Irene García-Maquilón, José Brea, Rocío Piña, Armando Albert, Pedro L. Rodriguez et al.
      Pages 99-111

    4. A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling

      • Irene García-Maquilón, Pedro L. Rodriguez, Aditya S. Vaidya, Jorge Lozano-Juste
      Pages 113-121

    5. Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening

      • Chanung Park, Dong Hye Seo, Joseph Buckley, Gyeong Mee Yoon
      Pages 123-129

    6. Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors

      • Kazuo Soeno, Akiko Sato, Yukihisa Shimada
      Pages 131-144

  5. Target Identification and Confirmation Approaches

    1. Front Matter

      Pages 145-145
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    2. Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach

      • Dan-dan Liu, Chang Zou, Jianbin Zhang, Peng Gao, Yongping Zhu, Yuqing Meng et al.
      Pages 147-161

    3. Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays

      • Cecilia Rodriguez-Furlan, Glenn R. Hicks
      Pages 163-173

    4. Drug Affinity Responsive Target Stability (DARTS ) Assay to Detect Interaction Between a Purified Protein and a Small Molecule

      • Lei Huang, Diwen Wang, Chunhua Zhang
      Pages 175-182
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Keywords

About this book

This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community.


Editors and Affiliations

  • Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, USA

    Glenn R. Hicks

  • Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University, West Lafayette, USA

    Chunhua Zhang

Bibliographic Information

  • Book Title: Plant Chemical Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Glenn R. Hicks, Chunhua Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0954-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0953-8Published: 04 December 2020

  • Softcover ISBN: 978-1-0716-0956-9Published: 04 December 2021

  • eBook ISBN: 978-1-0716-0954-5Published: 03 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 196

  • Number of Illustrations: 14 b/w illustrations, 30 illustrations in colour

  • Topics: Plant Biochemistry, Plant Genetics and Genomics, Plant Sciences, Genetics and Genomics

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