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  • © 2021

Periodontal Pathogens

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2210)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. Methods for Bacterial Genetic Manipulation

    1. Front Matter

      Pages 1-1
    2. Genetic Manipulations of Oral Spirochete Treponema denticola

      • Kurni Kurniyati, Chunhao Li
      Pages 15-23
    3. Construction of a Gene-Deletion Mutant in Tannerella forsythia

      • Keiji Nagano, Yoshiaki Hasegawa
      Pages 25-31
    4. Genetic Transformation of Fusobacterium nucleatum

      • Akihiro Yoshida, Akihiko Ikegami
      Pages 43-50
  3. Experimental Methods to Examine Virulence Factors

    1. Front Matter

      Pages 51-51
    2. Genotyping of Porphyromonas gingivalis in Relationship to Virulence

      • Atsuo Amano, Youn-Hee Choi, Hiroki Takeuchi
      Pages 53-59
    3. Transport and Polymerization of Porphyromonas gingivalis Type V Pili

      • Mikio Shoji, Satoshi Shibata, Mariko Naito, Koji Nakayama
      Pages 61-73
    4. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

      • Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, Richard J. Lamont
      Pages 75-86
    5. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

      • Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, Eric C. Reynolds
      Pages 113-121
    6. Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins

      • Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, Ashu Sharma
      Pages 135-142
    7. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

      • Yukitaka Murakami, Keiji Nagano, Yoshiaki Hasegawa
      Pages 143-155
    8. Characterization of the Treponema denticola Virulence Factor Dentilisin

      • Yuichiro Kikuchi, Kazuyuki Ishihara
      Pages 173-184

About this book

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Editors and Affiliations

  • Division of Microbiology, Department of Oral Biology School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan

    Keiji Nagano

  • Department of Microbiology School of Dentistry, Aichi Gakuin University, Nisshin, Japan

    Yoshiaki Hasegawa

Bibliographic Information

  • Book Title: Periodontal Pathogens

  • Book Subtitle: Methods and Protocols

  • Editors: Keiji Nagano, Yoshiaki Hasegawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0939-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0938-5Published: 20 August 2020

  • Softcover ISBN: 978-1-0716-0941-5Published: 20 August 2021

  • eBook ISBN: 978-1-0716-0939-2Published: 20 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 256

  • Number of Illustrations: 27 b/w illustrations, 31 illustrations in colour

  • Topics: Microbiology, Dentistry, Biomedicine, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access