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Polypeptide Materials

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Maxim G. Ryadnov

    1. National Physical Laboratory, Teddington, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step details essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2208)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. A Shortcut to the Synthesis of Peptide Thioesters

    • Richard Raz, John Offer
    Pages 1-12

  3. SnoopLigase-Mediated Peptide–Peptide Conjugation and Purification

    • Can M. Buldun, Irsyad N. A. Khairil Anuar, Mark Howarth
    Pages 13-31

  4. Peptide Nanoparticles for Gene Packaging and Intracellular Delivery

    • Paula Vila-Gómez, James E. Noble, Maxim G. Ryadnov
    Pages 33-48

  5. Synthesis and Application of Peptide–siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung

    • Jade J. Welch, David A. Dean, Bradley L. Nilsson
    Pages 49-67

  6. FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

    • Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, Jonathan G. Heddle
    Pages 69-80

  7. Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes

    • Irene Marzuoli, Franca Fraternali
    Pages 81-100

  8. Functional Peptide Nanocapsules Self-Assembled from β-Annulus Peptides

    • Hiroshi Inaba, Kazunori Matsuura
    Pages 101-121

  9. Electrostatic Self-Assembly of Protein Cage Arrays

    • Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, Mauri A. Kostiainen
    Pages 123-133

  10. Design and Generation of Self-Assembling Peptide Virus-like Particles with Intrinsic GPCR Inhibitory Activity

    • Sergey G. Tarasov, Marzena Dyba, Joshua Yu, Nadya Tarasova
    Pages 135-148

  11. Imaging and 3D Reconstruction of De Novo Peptide Capsids

    • Emiliana De Santis, Maxim G. Ryadnov
    Pages 149-165

  12. Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra

    • Abeer Alghamdi, Li Hung C. Chung, Olaf J. Rolinski
    Pages 167-177

  13. Forming Low-Molecular-Weight Hydrogels by Electrochemical Methods

    • Emily R. Cross, Kate McAulay, Dave J. Adams
    Pages 179-188

  14. In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study

    • Tristan O. C. Kwan, Rosana Reis, Isabel Moraes
    Pages 189-202

  15. Measurement of Peptide Coating Thickness and Chemical Composition Using XPS

    • David J. H. Cant, Alexander G. Shard, Caterina Minelli
    Pages 203-224

  16. Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy

    • Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom et al.
    Pages 225-235

  17. Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials

    • Jehangir Cama, Stefano Pagliara
    Pages 237-253

  18. Ultramicrotomy Analysis of Peptide-Treated Cells

    • Stephanie Rey, Nilofar Faruqui, Maxim G. Ryadnov
    Pages 255-264

  19. Back Matter

    Pages 265-268
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Keywords

About this book

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

Editors and Affiliations

  • National Physical Laboratory, Teddington, UK

    Maxim G. Ryadnov

Bibliographic Information

  • Book Title: Polypeptide Materials

  • Book Subtitle: Methods and Protocols

  • Editors: Maxim G. Ryadnov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0928-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0927-9Published: 28 August 2020

  • Softcover ISBN: 978-1-0716-0930-9Published: 28 August 2021

  • eBook ISBN: 978-1-0716-0928-6Published: 27 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 268

  • Number of Illustrations: 14 b/w illustrations, 66 illustrations in colour

  • Topics: Protein Science, Chemistry/Food Science, general, Biomaterials

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