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Biophysics of Membrane Proteins

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Vincent L. G. Postis

    1. School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK

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  2. Adrian Goldman

    1. Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences; Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Leeds, Leeds, UK; University of Helsinki, Helsinki, Finland

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2168)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Biochemistry and Functional Analysis

    1. Front Matter

      Pages 1-1
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    2. Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells

      • Yixin Liu, Ana Pavić, Joshua T. Farley, Carine de Marcos Lousa, Adrian Goldman, Vincent L. G. Postis
      Pages 3-49

    3. Quantifying the Interaction of Phosphite with ABC Transporters : MicroScale Thermophoresis and a Novel His-Tag Labeling Approach

      • Tanja Bartoschik, Amit Gupta, Beate Kern, Andrew Hitchcock, Nathan B. P. Adams, Nuska Tschammer
      Pages 51-62

    4. Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes

      • Dhenesh Puvanendran, Hager Souabni, Dimitri Salvador, Olivier Lambert, Quentin Cece, Martin Picard
      Pages 63-72

    5. Functional Characterization of SLC Transporters Using Solid Supported Membranes

      • Andre Bazzone, Maria Barthmes
      Pages 73-103

    6. Thermostability Assays: a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents

      • Steven P. D. Harborne, Martin S. King, Edmund R. S. Kunji
      Pages 105-121

    7. Direct Monitoring of GPCR Reconstitution and Ligand-Binding Activity by Plasmon Waveguide Resonance

      • Isabel D. Alves
      Pages 123-143

  3. Experimental and Theoretical Structural Determination

    1. Front Matter

      Pages 145-145
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    2. Examining Membrane Proteins by Neutron Scattering

      • Christine Ebel, Cécile Breyton, Anne Martel
      Pages 147-175

    3. Solution X-Ray Scattering for Membrane Proteins

      • Maciej Baranowski, Javier Pérez
      Pages 177-197

    4. Interpreting SAXS /WAXS Data with Explicit-Solvent Simulations: A Practical Guide

      • Markus R. Hermann, Jochen S. Hub
      Pages 199-215

    5. Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers

      • Gerard H. M. Huysmans, Dagan C. Marx, Sheena E. Radford, Karen G. Fleming
      Pages 217-232

    6. Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry

      • Dietmar Hammerschmid, Jeroen F. van Dyck, Frank Sobott, Antonio N. Calabrese
      Pages 233-261

    7. Determination of the Molecular Mass of Membrane Proteins Using Size-Exclusion Chromatography with Multiangle Laser Light Scattering (SEC-MALLS )

      • Maren Thomsen
      Pages 263-269

  4. Membrane Protein Dynamics and Conformations

    1. Front Matter

      Pages 271-271
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    2. Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales

      • Tomas Fessl, Joel A. Crossley, Daniel Watkins, Marek Scholz, Matthew A. Watson, Tara Sabir et al.
      Pages 273-297

    3. Short-Range Distance Measurement by Transition Metal Ion FRET

      • Jonas S. Mortensen, Claus J. Loland
      Pages 299-311

    4. PELDOR/DEER: An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers

      • Martin F. Peter, Kiran Bountra, Konstantinos Beis, Gregor Hagelueken
      Pages 313-333

  5. Back Matter

    Pages 335-336
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Keywords

About this book

This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.

Editors and Affiliations

  • School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK

    Vincent L. G. Postis

  • Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences; Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Leeds, Leeds, UK; University of Helsinki, Helsinki, Finland

    Adrian Goldman

Bibliographic Information

  • Book Title: Biophysics of Membrane Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Vincent L. G. Postis, Adrian Goldman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0724-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0723-7Published: 14 February 2021

  • Softcover ISBN: 978-1-0716-0726-8Published: 14 February 2022

  • eBook ISBN: 978-1-0716-0724-4Published: 13 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 336

  • Number of Illustrations: 12 b/w illustrations, 61 illustrations in colour

  • Topics: Membrane Biology, Biological and Medical Physics, Biophysics, Protein Science

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