Cardiac Regeneration

1. Front Matter

   Pages i-xiii

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2. Cardiac Injury Models

   1. Front Matter

      Pages 1-1

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   2. Myocardial Infarction Techniques in Adult Mice

      • Elad Bassat, Dahlia E. Perez, Eldad Tzahor

      Pages 3-21

   3. Apical Resection and Cryoinjury of Neonatal Mouse Heart

      • Hua Shen, Ali Darehzereshki, Henry M. Sucov, Ching-Ling Lien

      Pages 23-32

   4. Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice

      • Tilman Ziegler, Karl-Ludwig Laugwitz, Christian Kupatt

      Pages 33-41

   5. Myocardial Infarction in Pigs

      • Andrea Bähr, Nadja Hornaschewitz, Christian Kupatt

      Pages 43-50

   6. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

      • Ines J. Marques, Andrés Sanz-Morejón, Nadia Mercader

      Pages 51-62Open Access

   7. Cardiac Resection Injury in Zebrafish

      • Delicia Z. Sheng, Dawei Zheng, Kazu Kikuchi

      Pages 63-69

   8. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

      • Fei Sun, Adam R. Shoffner, Kenneth D. Poss

      Pages 71-80

   9. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration

      • Yijen L. Wu

      Pages 81-106

3. Ex Vivo and In Vivo Approaches

   1. Front Matter

      Pages 107-107

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   2. Isolation, Culture, and Live-Cell Imaging of Primary Rat Cardiomyocytes

      • Marina Leone, Felix B. Engel

      Pages 109-124

   3. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

      • Lu Han, Jocelyn Mich-Basso, Bernhard Kühn

      Pages 125-139

   4. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells

      • Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, Ravi Karra

      Pages 141-153

   5. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells

      • Jun Xu, Li Wang, Jiandong Liu, Li Qian

      Pages 155-170

   6. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs

      • Abbigail Helfer, Nenad Bursac

      Pages 171-186

   7. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells

      • Ling Gao, Jianyi (Jay) Zhang

      Pages 187-197

   8. Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts

      • Honghai Liu, Kevin Bersell, Bernhard Kühn

      Pages 199-210

   9. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

      • Sierra Duca, Jingli Cao

      Pages 211-222

   10. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters

       • Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, Sean M. Wu

       Pages 223-240

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models andmethods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.


The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

• Myocardial infarction
• Cardiomyocytes
• Cardiac injury
• Innate heart regeneration
• Animal models

• Department of Cell Biology, Duke University Medical Center, Durham, USA

  Kenneth D. Poss

• Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, USA

  Bernhard Kühn

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