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Expression, Purification, and Structural Biology of Membrane Proteins

  • Book
  • © 2020

Overview

Editors:
  1. Camilo Perez

    1. Biozentrum, University of Basel, Basel, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Timm Maier

    1. Biozentrum, University of Basel, Basel, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2127)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae

    • Porsha L. R. Shaw, Kathryn A. Diederichs, Ashley Pitt, Sarah E. Rollauer, Susan K. Buchanan
    Pages 1-11

  3. Membrane Protein Production in Escherichia coli

    • Benjamin C. McIlwain, Ali A. Kermani
    Pages 13-27

  4. Membrane Protein Production in Lactococcus lactis for Structural Studies

    • Chloe Martens
    Pages 29-45

  5. Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae

    • Martin S. King, Edmund R. S. Kunji
    Pages 47-61

  6. Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System

    • Jacques Boivineau, Matthias Haffke, Veli-Pekka Jaakola
    Pages 63-80

  7. Membrane Extracts from Plant Tissues

    • Uwe Bodensohn, Christoph Ruland, Roman Ladig, Enrico Schleiff
    Pages 81-92

  8. Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter

    • Charlott Stock, Inga Hänelt
    Pages 93-103

  9. GPCR Solubilization and Quality Control

    • Tamara Miljus, David A. Sykes, Clare R. Harwood, Ziva Vuckovic, Dmitry B. Veprintsev
    Pages 105-127

  10. Affinity Purification of Membrane Proteins

    • Elisabeth Graeber, Volodymyr M. Korkhov
    Pages 129-137

  11. Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage

    • Stephan Hirschi, Dimitrios Fotiadis
    Pages 139-150

  12. Biotinylation of Membrane Proteins for Binder Selections

    • Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A. J. Hutter, Christian Miscenic et al.
    Pages 151-165Open Access

  13. Production and Application of Nanobodies for Membrane Protein Structural Biology

    • Janine Denise Brunner, Stephan Schenck
    Pages 167-184

  14. Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay

    • Peter Stohler, Roger J. P. Dawson
    Pages 185-190

  15. Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses

    • Philipp A. M. Schmidpeter, Nattakan Sukomon, Crina M. Nimigean
    Pages 191-205

  16. Reconstitution of Proteoliposomes for Phospholipid Scrambling and Nonselective Channel Assays

    • Maria E. Falzone, Alessio Accardi
    Pages 207-225

  17. Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent

    • Magnus Bloch, Mònica Santiveri, Nicholas M. I. Taylor
    Pages 227-244

  18. Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs

    • Valeria Kalienkova, Carolina Alvadia, Vanessa Clerico Mosina, Cristina Paulino
    Pages 245-273

  19. Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy

    • Natalie Bärland, Camilo Perez
    Pages 275-282

  20. Stabilization and Crystallization of a Membrane Protein Involved in Lipid Transport

    • Bing Zhang, Camilo Perez
    Pages 283-292
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Keywords

About this book

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins.


Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.

Editors and Affiliations

  • Biozentrum, University of Basel, Basel, Switzerland

    Camilo Perez, Timm Maier

Bibliographic Information

  • Book Title: Expression, Purification, and Structural Biology of Membrane Proteins

  • Editors: Camilo Perez, Timm Maier

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0373-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0372-7Published: 29 February 2020

  • Softcover ISBN: 978-1-0716-0375-8Published: 29 February 2020

  • eBook ISBN: 978-1-0716-0373-4Published: 28 February 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 423

  • Number of Illustrations: 11 b/w illustrations, 78 illustrations in colour

  • Topics: Protein Science, Membrane Biology

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