Stem Cell Transcriptional Networks
Methods and Protocols
Herausgeber: Kidder, Benjamin (Ed.)
- includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.
- Inhaltsverzeichnis (19 Kapitel)
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Epitope Tagging ChIP-Seq of DNA Binding Proteins Using CETCh-Seq
Seiten 3-34
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User-Friendly and Interactive Analysis of ChIP-Seq Data Using EaSeq
Seiten 35-63
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Evaluation of 3D Chromatin Interactions Using Hi-C
Seiten 65-78
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MSTD for Detecting Topological Domains from 3D Genomic Maps
Seiten 79-92
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Creating 2D Occupancy Plots Using plot2DO
Seiten 93-108
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Inhaltsverzeichnis (19 Kapitel)
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Bibliografische Information
- Bibliographic Information
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- Buchtitel
- Stem Cell Transcriptional Networks
- Buchuntertitel
- Methods and Protocols
- Herausgeber
-
- Benjamin Kidder
- Titel der Buchreihe
- Methods in Molecular Biology
- Buchreihen Band
- 2117
- Copyright
- 2020
- Verlag
- Springer US
- Copyright Inhaber
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0301-7
- DOI
- 10.1007/978-1-0716-0301-7
- Hardcover ISBN
- 978-1-0716-0300-0
- Softcover ISBN
- 978-1-0716-0303-1
- Buchreihen ISSN
- 1064-3745
- Auflage
- 2
- Seitenzahl
- XI, 308
- Anzahl der Bilder
- 8 schwarz-weiß Abbildungen, 67 Abbildungen in Farbe
- Themen
