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Targeting Enzymes for Pharmaceutical Development

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Nikolaos E. Labrou

    1. Department of Biotechnology, Agricultural University of Athens, Athens, Greece

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2089)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. In Silico Laboratory: Tools for Similarity-Based Drug Discovery

    • Samo LeÅ¡nik, Janez Konc
    Pages 1-28

  3. The In Silico Fischer Lock-and-Key Model: The Combined Use of Molecular Descriptors and Docking Poses for the Repurposing of Old Drugs

    • Marco Tutone, Anna Maria Almerico
    Pages 29-39

  4. Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor

    • Nikolaos Georgakis, Elisavet Ioannou, Christina Varotsou, Georgios Premetis, Evangelia G. Chronopoulou, Nikolaos E. Labrou
    Pages 41-46

  5. Applications of Differential Scanning Fluorometry and Related Technologies in Characterization of Protein–Ligand Interactions

    • Bolormaa Baljinnyam, Michael Ronzetti, Adam Yasgar, Anton Simeonov
    Pages 47-68

  6. High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins

    • Alanna E. Sorenson, Patrick M. Schaeffer
    Pages 69-85

  7. Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism

    • Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
    Pages 87-118Open Access

  8. Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance

    • Steven W. Polyak, Rumana Mowla, Henrietta Venter
    Pages 119-130

  9. Systematic Screening of Viral Entry Inhibitors Using Surface Plasmon Resonance

    • Penmetcha K. R. Kumar
    Pages 131-145

  10. Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry

    • Jan Schejbal, Roman Řemínek, ZdenÄ›k Glatz
    Pages 147-157

  11. Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA)

    • Alanna E. Sorenson, Patrick M. Schaeffer
    Pages 159-166

  12. Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors

    • Roman Řemínek, ZdenÄ›k Glatz, Wolfgang Thormann
    Pages 167-178

  13. Enzymatic Bioautographic Methods

    • I. Ayelen Ramallo, Mario O. Salazar, Ricardo L. E. Furlan
    Pages 179-189

  14. High-Throughput Assessment of Metabolism-Induced Toxicity of Compounds on a 384-Pillar Plate

    • Soo-Yeon Kang, Kyeong-Nam Yu, Pranav Joshi, Moo-Yeal Lee
    Pages 191-207

  15. Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors

    • Abraham Ochoa, Frida Trejo, Luis F. Olguín
    Pages 209-233

  16. Ligand Fishing: An Approach for the Discovery of Inhibitors from Complex Biological Mixtures

    • Evangelia G. Chronopoulou, Christina Varotsou, Nikolaos Georgakis, Georgios Premetis, Elisavet Ioannou, Nikolaos E. Labrou
    Pages 235-243

  17. HMG-CoA Reductase as Target for Drug Development

    • Baskaran Gunasekaran, Mohd Yunus Shukor
    Pages 245-250

  18. Lipoxygenases as Targets for Drug Development

    • Xiao-Yuan Mao
    Pages 251-256

  19. Cholinesterase as a Target for Drug Development in Alzheimer’s Disease

    • Piyoosh Sharma, Manish Kumar Tripathi, Sushant Kumar Shrivastava
    Pages 257-286

  20. Correction To: Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism

    • Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
    Pages C1-C1
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Keywords

About this book

This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols serves as a vital reference for academics and industry professionals working on expanding our understanding of the wide range of important enzyme targets.

Editors and Affiliations

  • Department of Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

Bibliographic Information

  • Book Title: Targeting Enzymes for Pharmaceutical Development

  • Book Subtitle: Methods and Protocols

  • Editors: Nikolaos E. Labrou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0163-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2020

  • Hardcover ISBN: 978-1-0716-0162-4Published: 27 November 2019

  • Softcover ISBN: 978-1-0716-0165-5Published: 27 November 2020

  • eBook ISBN: 978-1-0716-0163-1Published: 26 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 290

  • Number of Illustrations: 42 b/w illustrations, 71 illustrations in colour

  • Topics: Pharmaceutical Sciences/Technology, Enzymology, Medicinal Chemistry

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