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Techniques in Molecular Biology

Volume 2

  • Book
  • © 1987

Overview

Editors:
  1. John M. Walker

    1. Division of Biological and Environmental Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, England

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  2. Wim Gaastra

    1. Department of Infectious Diseases, Section of Bacteriology, University of Utrecht, Utrecht, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

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Table of contents (17 chapters)

  1. Front Matter

    Pages i-iv
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  2. Micromethods in Protein Chemistry

    • Elaine L. V. Harris
    Pages 1-31

  3. Peptide Synthesis

    • D. T. Elmore
    Pages 32-54

  4. Affinity Chromatography

    • S. J. Burton
    Pages 55-81

  5. The Enzyme Linked Immunosorbent Assay (ELISA)

    • John M. Walker
    Pages 82-97

  6. Monoclonal Antibodies

    • John N. Wood
    Pages 98-110

  7. Plasmid Derived Cloning Vectors

    • Wim Gaastra, Hans E. N. Bergmans
    Pages 111-126

  8. The Ti-Plasmid of Agrobacterium Tumefaciens as a Tool for Genetic Engineering in Plants

    • Wim Gaastra, Kirsten Hansen
    Pages 127-139

  9. Cloning in Yeast

    • Elliot B. Gingold
    Pages 140-158

  10. Cloning in Bacteriophage Lambda

    • Jeremy W. Dale
    Pages 159-177

  11. The Dot-Blot Technique

    • Paul D. van Helden, C. Louise Olliver
    Pages 178-186

  12. DNA-Mediated Gene Transfer into Mammalian Cells

    • Roger F. L. James, Frank G. Grosveld
    Pages 187-202

  13. In Vitro Transcription

    • Robert J. Slater
    Pages 203-227

  14. Chemical Synthesis of Oligodeoxyribonucleotides

    • H. A. White
    Pages 228-250

  15. Targeted Mutagenesis of Cloned DNA

    • Bé Wieringa
    Pages 251-273

  16. DNA Footprinting and Related Techniques for Analysing Protein-DNA Interactions

    • Miguel Beato
    Pages 274-287

  17. Autoradiography and Fluorography

    • Stephen A. Boffey
    Pages 288-295

  18. Computer Applications to Studying DNA

    • Robert Harr, Petter Gustafsson
    Pages 296-317

  19. Back Matter

    Pages 318-332
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Keywords

About this book

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro­ duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con­ tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro­ duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method­ ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin­ ology, a glossary of terms has been included.

Editors and Affiliations

  • Division of Biological and Environmental Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, England

    John M. Walker

  • Department of Infectious Diseases, Section of Bacteriology, University of Utrecht, Utrecht, The Netherlands

    Wim Gaastra

Bibliographic Information

  • Book Title: Techniques in Molecular Biology

  • Book Subtitle: Volume 2

  • Editors: John M. Walker, Wim Gaastra

  • DOI: https://doi.org/10.1007/978-1-4615-9799-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: John M. Walker and Wim Gaastra 1987

  • Softcover ISBN: 978-0-7099-3673-2Published: 22 January 1987

  • eBook ISBN: 978-1-4615-9799-5Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: IV, 332

  • Number of Illustrations: 16 b/w illustrations

  • Topics: Biochemistry, general, Science, Humanities and Social Sciences, multidisciplinary

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