Protein Purification

1. Front Matter

   Pages i-xix

   PDF

2. The Protein Purification Laboratory

   • Robert K. Scopes

   Pages 1-21

3. Making an Extract

   • Robert K. Scopes

   Pages 22-43

4. Analysis—Measurement of Protein and Enzyme Activity

   • Robert K. Scopes

   Pages 44-70

5. Separation by Precipitation

   • Robert K. Scopes

   Pages 71-101

6. Separation by Adsorption I: General Principles

   • Robert K. Scopes

   Pages 102-145

7. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents

   • Robert K. Scopes

   Pages 146-186

8. Separation by Adsorption—Affinity Techniques

   • Robert K. Scopes

   Pages 187-237

9. Separation in Solution

   • Robert K. Scopes

   Pages 238-269

10. Purification of Special Types of Proteins

    • Robert K. Scopes

    Pages 270-282

11. Small-Scale and Large-Scale Procedures

    • Robert K. Scopes

    Pages 283-292

12. Analysis for Purity

    • Robert K. Scopes

    Pages 293-309

13. Optimization of Procedures; Final Steps

    • Robert K. Scopes

    Pages 310-345

14. Back Matter

    Pages 346-380

    PDF

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc­ tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

• Enzymreindarstellung
• biochemistry
• chemistry
• enzyme
• enzymes
• protein

From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)

 

• Department of Biochemistry and the Centre for Protein and Enzyme Technology, La Trobe University, Bundoora, Australia

  Robert K. Scopes

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