Comprehensive Enzyme Kinetics

1. Front Matter

   Pages i-xi

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2. Introduction

   Pages 1-10

3. Chemical Kinetics

   Pages 11-30

4. Kinetics of Monosubstrate Reactions

   Pages 31-49

5. Derivation of Rate Equations

   Pages 51-71

6. Linear Inhibition

   Pages 73-94

7. Hyperbolic and Parabolic Inhibition

   Pages 95-110

8. Enzyme Activation

   Pages 111-116

9. Kinetics of Rapid Equilibrium Bisubstrate Reactions

   Pages 117-138

10. Steady-State Kinetics of Bisubstrate Reactions

    Pages 139-170

11. Kinetic Analysis of Bisubstrate Mechanisms

    Pages 171-189

12. Substrate Inhibition and Mixed Dead-End and Product Inhibition

    Pages 191-207

13. Trisubstrate Mechanisms

    Pages 209-241

14. Cooperative and Allosteric Effects

    Pages 243-282

15. The pH Dependence of Enzyme Catalysis

    Pages 283-315

16. Effects of Temperature on Enzyme Reactions

    Pages 317-327

17. Isotope Exchange

    Pages 329-351

18. Kinetic Isotope Effects

    Pages 353-390

19. Statistical Analysis of Initial Rate and Binding Data

    Pages 391-419

20. Back Matter

    Pages 421-438

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Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

• Isotop
• biochemistry
• biology
• catalysis
• enzyme
• enzymes
• kinetics
• molecular biology
• research

`As scientific attention turns in the post-genomic era, once again, to the properties of proteins, and particularly to their preeminent role as catalysts, Professor Leskovac's book, Comprehensive Enzyme Kinetics, will have to be on every shelf. It will, however, rarely find itself on the shelf, but much more in the hands of researchers who will daily deploy in the laboratory its wealth of useful information. Prepared under the eye of W.W. Cleland, the demiurge of enzyme kinetics, this magnum opus earns its title of comprehensive. The systematic organization of the volume, which somehow manages completeness in just over 400 pages, leads the reader to a target subject easily. The clarity of exposition will be as welcome to the experienced kineticist as it will be to the beginner. This volume is a singular achievement.'
R.L. Schowen, Professor Emeritus Chemistry, Molecular Biosciences and Pharmaceutical Chemistry, University of Kansas
`Comprehensive Enzyme Kinetics is an excellent addition to the enzyme kinetics literature. It touches on all of the topics in the various areas of kinetics that might be of interest to the enzymologist. It will make a fine classroom reference text.'
Herbert J. Fromm, Distinguished Professor, Iowa State University

• University of Novi Sad, Novi Sad, Serbia

  Vladimir Leskovac

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