Springer Theses

Artificial Assemblies with Cooperative DNA Recognition

Authors: Yu, Zutao

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  • Nominated as an outstanding PhD thesis by Kyoto University 
  • Is the first-ever report on three elegant designs of cooperative DNA-binding systems 
  • Highlights the most important aspects of programmable DNA-binding pyrrole–imidazole polyamides for quick learning 
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eBook 106,99 €
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  • ISBN 978-981-15-4423-1
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Hardcover 129,99 €
price for China (P.R.) (gross)
  • ISBN 978-981-15-4422-4
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About this book

This book presents three types of synthetically cooperative DNA recognizing assemblies, in order to advance the development of programmable DNA-binding pyrrole–imidazole polyamides (PIPs). 

PIPs represent the best-characterized class of small molecule DNA binders that can be modified to bind with any predetermined DNA sequence and regulate gene expression patterns in a transgene-free and cost-effective manner. PIPs are characterized by their small molecular size, high binding affinity, programmability, sequence selectivity, and moderate cell permeability. In recent years, there have been numerous novel studies on the applications of these biological tools; this research is thoroughly reviewed in the first chapter.   

There are several critical issues, however, that impede the further broad study of PIPs, which greatly concern the author. For instance, the short PIP version has an excessively hi^10 bp; this significantly decreases cell permeability. Moreover, the conventional binding strategy for PIP design cannot apply to flexible DNA binding—for example, the DNA-binding mode of a transcription factor pair. 

In this book, the author describes the development of three kinds of cooperative DNA-binding systems that help resolve the current highly problematic issues concerning PIPs. These three systems offer a range of significant advantages, such as favorable sequence selectivity, long recognition sequence, higher binding affinity, and a flexible gap distance. 

Released at a critical juncture in the application of PIPs, this book will greatly facilitate their use as therapeutic drugs in the treatment of cancer and hereditary diseases, and in regenerative medicine. 

Table of contents (4 chapters)

Table of contents (4 chapters)
  • Synthetic DNA Binding Assembly: Architecture, Application and Perspectives

    Pages 1-39

    YU, Zutao

  • PIP–HoGu, an Artificial Assembly with Cooperative DNA Recognition

    Pages 41-76

    YU, Zutao

  • PIP–NaCo, a Synergic DNA Binding System Assisted by Orthogonal γPNA Dimerization Domains with Cooperativity and Versatility

    Pages 77-103

    YU, Zutao

  • ePIP–HoGu, A Cooperative DNA Binding System to Recruit Epigenetic Modifier to the Targeted DNA Locus

    Pages 105-133

    YU, Zutao

Buy this book

eBook 106,99 €
price for China (P.R.) (gross)
  • ISBN 978-981-15-4423-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 129,99 €
price for China (P.R.) (gross)
  • ISBN 978-981-15-4422-4
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Artificial Assemblies with Cooperative DNA Recognition
Authors
Series Title
Springer Theses
Copyright
2020
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-15-4423-1
DOI
10.1007/978-981-15-4423-1
Hardcover ISBN
978-981-15-4422-4
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XV, 136
Number of Illustrations
17 b/w illustrations, 119 illustrations in colour
Topics

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