NMR-Based Metabolomics

1. Front Matter

   Pages i-xiv

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2. Historical and Methodological Overview

   1. Front Matter

      Pages 1-1

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   2. Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges

      • G. A. Nagana Gowda, Daniel Raftery

      Pages 3-14

3. Metabolite Analysis Methods

   1. Front Matter

      Pages 15-15

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   2. Analysis of Plasma, Serum, and Whole Blood Metabolites Using 1H NMR Spectroscopy

      • G. A. Nagana Gowda, Daniel Raftery

      Pages 17-34

   3. Lipid Profiling Using 1H NMR Spectroscopy

      • Miriam Gil, Sara Samino, Rubén Barrilero, Xavier Correig

      Pages 35-47

   4. High-Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics

      • Marlon Tilgner, Tim S. Vater, Piet Habbel, Leo L. Cheng

      Pages 49-67

   5. Practical Guidelines for 13C-Based NMR Metabolomics

      • Arthur S. Edison, Adrien Le Guennec, Frank Delaglio, Ēriks Kupče

      Pages 69-95

   6. Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D 1H NMR Spectroscopy

      • G. A. Nagana Gowda, Daniel Raftery

      Pages 97-110

4. Unknown Resonance Identification

   1. Front Matter

      Pages 111-111

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   2. UHPLC-QTOF-MS/MS-SPE-NMR: A Solution to the Metabolomics Grand Challenge of Higher-Throughput, Confident Metabolite Identifications

      • Anil Bhatia, Saurav J. Sarma, Zhentian Lei, Lloyd W. Sumner

      Pages 113-133

   3. Fast NMR Methods for Identification of Resonances and Metabolic Pathways

      • Abhinav Dubey, Shivanand M. Pudakalakatti, Hanudatta S. Atreya

      Pages 135-147

5. Stable Isotope-Resolved NMR

   1. Front Matter

      Pages 149-149

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   2. Stable Isotope-Resolved Metabolomics by NMR

      • Penghui Lin, Andrew N. Lane, Teresa W.-M. Fan

      Pages 151-168

   3. Tracking Metabolic Rewiring of Cancer Metabolism in Humans Using Isotope-Resolved NMR

      • Kumar Pichumani

      Pages 169-186

6. Biomarker Discovery

   1. Front Matter

      Pages 187-187

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   2. NMR-Based Metabolomics in Cardiac Research

      • Julian L. Griffin, Cecilia Castro

      Pages 189-194

   3. Breast Cancer Metabolomics Using NMR

      • Uma Sharma, Naranamangalam R. Jagannathan

      Pages 195-213

   4. NMR-Based Urinary Metabolomics Applications

      • Tedros Bezabeh, Ana Capati, Omkar B. Ijare

      Pages 215-229

   5. NMR-Based Metabolomics in Gallbladder Cancer Research

      • Renuka Ranjan, Neeraj Sinha

      Pages 231-241

This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advancesin the field of metabolomics.

• Nuclear magnetic resonance spectroscopy
• Metabolite analysis
• Biological samples
• Biomarker discovery
• Drug discovery
• Data analysis
• Metabolomics

• Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, USA

  G. A. Nagana Gowda

• Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Fred Hutch Cancer Research Center, Seattle, USA

  Daniel Raftery

Policies and ethics