Skip to main content
  • Book
  • © 2019

Neural Stem Cells

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1919)

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
  2. Making NSC and Neurons from Patient-Derived Tissue Samples

    • Odity Mukherjee, Shubhra Acharya, Mahendra Rao
    Pages 9-24
  3. Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

    • Mohamad Khazaei, Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, Michael G. Fehlings
    Pages 25-41
  4. Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells

    • Rodolfo Gonzalez, Ibon Garitaonandia, Andrey Semechkin, Russell Kern
    Pages 43-57
  5. Chemically Defined Neural Conversion of Human Pluripotent Stem Cells

    • Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov et al.
    Pages 59-72
  6. In Vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology

    • Marita Grønning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, Zaal Kokaia
    Pages 73-88
  7. Midbrain Dopaminergic Neurons Differentiated from Human-Induced Pluripotent Stem Cells

    • Fabiano Araújo Tofoli, Ana Teresa Silva Semeano, Ágatha Oliveira-Giacomelli, Maria Carolina Bittencourt Gonçalves, Merari F. R. Ferrari, Lygia Veiga Pereira et al.
    Pages 97-118
  8. Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells

    • Jeffrey Kim, Marcel M. Daadi
    Pages 129-143
  9. Reference Transcriptome for Deriving Marmoset Induced Pluripotent Stem Cells

    • Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav Roy-Choudhury, Jeffrey Kim et al.
    Pages 175-186
  10. Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach

    • Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, Peter J. Hornsby
    Pages 187-197
  11. Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells

    • Hyenjong Hong, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi
    Pages 199-204
  12. Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation

    • Cang Chen, Michael J. Guderyon, Guo Ge, Robert A. Clark, Senlin Li
    Pages 205-213
  13. Central and Peripheral Secondary Cell Death Processes after Transient Global Ischemia in Nonhuman Primate Cerebellum and Heart

    • Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert et al.
    Pages 215-225
  14. Method for Stimulation of Hippocampal Neurogenesis by Transient Microneedle Insertion

    • Shijie Song, Xiaoyung Kong, Juan Sanchez-Ramos
    Pages 227-235
  15. Back Matter

    Pages 237-239

About this book

This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, USA

    Marcel M. Daadi

Bibliographic Information

  • Book Title: Neural Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Marcel M. Daadi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9007-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9005-4Published: 18 January 2019

  • eBook ISBN: 978-1-4939-9007-8Published: 17 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 239

  • Number of Illustrations: 4 b/w illustrations, 47 illustrations in colour

  • Topics: Neurosciences, Stem Cells, Animal Models

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access