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Phytoplasmas

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Rita Musetti

    1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Laura Pagliari

    1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1875)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
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  2. Phytoplasmas: An Introduction

    • L. Pagliari, R. Musetti
    Pages 1-6

  3. Preparing Plant Material

    1. Front Matter

      Pages 7-7
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    2. Micro-Tom Tomato Grafting for Stolbur-Phytoplasma Transmission: Different Grafting Techniques

      • Sara Buoso, Alberto Loschi
      Pages 9-19

    3. Phytoplasma Transmission: Insect Rearing and Infection Protocols

      • L. Pagliari, J. Chuche, D. Bosco, D. Thiéry
      Pages 21-36

    4. Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors

      • Kerstin Krüger, Nicola Fiore
      Pages 37-52

    5. Symptoms of Phytoplasma Diseases

      • Paolo Ermacora, Ruggero Osler
      Pages 53-67

  4. Molecular Analyses

    1. Front Matter

      Pages 69-69
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    2. Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas

      • Carmine Marcone
      Pages 71-81

    3. Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene

      • Assunta Bertaccini, Samanta Paltrinieri, Nicoletta Contaldo
      Pages 83-95

    4. PCR-Based Sequence Analysis on Multiple Genes Other than 16S rRNA Gene for Differentiation of Phytoplasmas

      • Marta Martini, Kristi D. Bottner-Parker, Ing-Ming Lee
      Pages 97-115

    5. Real-Time PCR Protocol for Phytoplasma Detection and Quantification

      • Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, Peter Abrahamian
      Pages 117-130

    6. Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in Its Host Plant without External Standard Curves

      • Sanja Baric
      Pages 131-141

    7. A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas

      • Shigeyuki Kakizawa
      Pages 143-149

    8. One-Step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas

      • Ioanna Malandraki, Christina Varveri, Nikon Vassilakos
      Pages 151-157

    9. A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification

      • Claudio Ratti, Stefano Minguzzi, Massimo Turina
      Pages 159-169

    10. Quantitative Analysis with Droplet Digital PCR

      • Nataša Mehle, Tanja Dreo
      Pages 171-186

    11. Rapid Sample Preparation and LAMP for Phytoplasma Detection

      • Jennifer Hodgetts
      Pages 187-201

    12. Assembly of Phytoplasma Genome Drafts from Illumina Reads Using Phytoassembly

      • Cesare Polano, Giuseppe Firrao
      Pages 203-211

    13. Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of “Flavescence Dorée”

      • H. Al-Saddik, A. Laybros, J. C. Simon, F. Cointault
      Pages 213-238
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Keywords

About this book

This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

 

Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Rita Musetti, Laura Pagliari

Bibliographic Information

  • Book Title: Phytoplasmas

  • Book Subtitle: Methods and Protocols

  • Editors: Rita Musetti, Laura Pagliari

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8837-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8836-5Published: 26 October 2018

  • Softcover ISBN: 978-1-4939-9400-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8837-2Published: 26 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 362

  • Number of Illustrations: 11 b/w illustrations, 64 illustrations in colour

  • Topics: Plant Sciences

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