Skip to main content
SpringerLink
Log in

Cell Polarity Signaling

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Chenbei Chang

    1. Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Jianbo Wang

    1. Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2438)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Use of Fluorescence Recovery After Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction–Associated Polarity Proteins

    • Samantha J. Warrington, Helen Strutt, David Strutt
    Pages 1-30

  3. FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs

    • Bryce T. Bajar, Xinmeng Guan, Amy Lam, Michael Z. Lin, Ryohei Yasuda, Tal Laviv et al.
    Pages 31-43

  4. Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics

    • Tejeshwar C. Rao, Tomasz J. Nawara, Alexa L. Mattheyses
    Pages 45-58

  5. Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates

    • Naomi Stolpner, Daniel J. Dickinson
    Pages 59-81

  6. Biochemical Assays to Detect Activation of Small GTPases Rho, Rac , and Cdc42 during Morphogenesis

    • Mark L. Berns, Raymond Habas
    Pages 83-95

  7. Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos

    • Ilya Chuykin, Sergei Y. Sokol
    Pages 97-106

  8. Regulation of Cell Polarity by Posttranslational Protein Palmitoylation

    • Baoen Chen, Carla Guarino, Abdelhalim Azzi, Hannah Erb, Xu Wu
    Pages 107-121

  9. Enrichment and Detection of Wnt Proteins from Cell Culture Media

    • Pooja R. Sonavane, Karl Willert
    Pages 123-131

  10. Using Live Imaging to Examine Early Cardiac Development in Zebrafish

    • Tess McCann, Rabina Shrestha, Alexis Graham, Joshua Bloomekatz
    Pages 133-145

  11. Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm

    • Olga Ossipova, Pamela Mancini, Sergei Y. Sokol
    Pages 147-161

  12. In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis

    • Bhargav D. Sanketi, Natasza A. Kurpios
    Pages 163-181

  13. Avian Embryos as a Model to Study Vascular Development

    • Bhargav D. Sanketi, Natasza A. Kurpios
    Pages 183-195

  14. BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice

    • Deli Yu, Jianbo Wang
    Pages 197-216

  15. Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics

    • Abigail R. Marshall, Eirini Maniou, Dale Moulding, Nicholas D. E. Greene, Andrew J. Copp, Gabriel L. Galea
    Pages 217-230

  16. Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo

    • Claudio Cortes, Christopher De Bono, Charlotte Thellier, Alexandre Francou, Robert G. Kelly
    Pages 231-250

  17. Methods to Investigate Cell Polarity of Inner Ear

    • Jihan Lyu, Xiaoqing Qian, Binjun Chen, Dongdong Ren
    Pages 251-275

  18. Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice

    • Kathryn M. Miller, Pau Marfull-Oromí, Yimin Zou
    Pages 277-286

  19. In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance

    • Pau Marfull-Oromí, Kathryn M. Miller, Yimin Zou
    Pages 287-301

  20. Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance

    • Pau Marfull-Oromí, Kathryn M. Miller, Yimin Zou
    Pages 303-308
Back to top

Keywords

About this book

This volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity. The collection includes the usage of a wide variety of model systems and an extensive array of techniques, including genetic, imaging, biochemical, and biomechanical. Written for the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Cell Polarity Signaling: Methods and Protocols aims to enable researchers to delve into the stimulating field of cell polarity and contribute to our understanding of how coordinated control of protein stability, trafficking, membrane retention, post-translational modification, and dynamic organization leads to active regulation of cell polarity. 


Chapter 29 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, USA

    Chenbei Chang, Jianbo Wang

Bibliographic Information

  • Book Title: Cell Polarity Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Chenbei Chang, Jianbo Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2035-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2034-2Published: 12 February 2022

  • Softcover ISBN: 978-1-0716-2037-3Published: 11 February 2022

  • eBook ISBN: 978-1-0716-2035-9Published: 11 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 530

  • Number of Illustrations: 19 b/w illustrations, 105 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation