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Stem Cell Assays

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Nagarajan Kannan

    1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Philip Beer

    1. Hull York Medical School, York, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2429)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. ES/iPS

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells

      • Xiugong Gao, Robert L. Sprando, Jeffrey J. Yourick
      Pages 3-14

    3. Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes

      • Daphne Norma Crasta, Satish Kumar Adiga, Nagarajan Kannan, Guruprasad Kalthur
      Pages 15-26

    4. Generation of Human iPSC from Small Volume Peripheral Blood Samples

      • Doreen Kloos, Nico Lachmann
      Pages 27-39

    5. Distinguishing Between Endodermal and Pluripotent Stem Cell Lines During Somatic Cell Reprogramming

      • A. Moauro, A. Ralston
      Pages 41-55

    6. Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

      • Alexander Keller, Nuša Krivec, Christina Markouli, Claudia Spits
      Pages 57-72

    7. Detection of Soluble and Insoluble Protein Species in Patient-Derived iPSCs

      • Stephanie Santarriaga, Ian Luecke, Allison D. Ebert
      Pages 73-84

    8. Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells

      • Enkhtuul Tsogtbaatar, Katherine Minter-Dykhouse, Alicia Saarinen, Clifford D. L. Folmes
      Pages 85-102

    9. Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions

      • Gene I. Uenishi, Ho Sun Jung, Igor I. Slukvin
      Pages 103-124

  3. ES/iPS Derived Tissue Stem Cells

    1. Front Matter

      Pages 125-125
      Download chapter PDF

    2. Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells

      • Rong Li, Yang Zhao, Jeffrey J. Yourick, Robert L. Sprando, Xiugong Gao
      Pages 127-142

    3. Differentiation of Human Induced Pluripotent Stem Cells into Cortical Neurons to Advance Precision Medicine

      • M. Catarina Silva, Ghata Nandi, Stephen J. Haggarty
      Pages 143-174

    4. Differentiation of iPS-Cells into Peripheral Sensory Neurons

      • Anika Neureiter, Esther Eberhardt, Angelika Lampert
      Pages 175-188

    5. Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitative Assays for Studying Axonal Phenotypes

      • Maria Giovanna Garone, Chiara D’Antoni, Alessandro Rosa
      Pages 189-199

    6. iPS Cell Differentiation into Brain Microvascular Endothelial Cells

      • Angelica Medina, Hengli Tang
      Pages 201-213

    7. Chromatin Immunoprecipitation in Human Pluripotent Stem Cell-Derived 3D Organoids to Analyze DNA–Protein Interactions

      • Wei Xuan Tan, Chek Mei Bok, Natasha Hui Jin Ng, Adrian Kee Keong Teo
      Pages 215-232

    8. Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells

      • Mengcheng Shen, Chun Liu, Joseph C. Wu
      Pages 233-246

    9. Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells

      • Junichi Tanaka, Kenji Mishima
      Pages 247-255

    10. In Vitro Generation of Heart Field Specific Cardiomyocytes

      • Arash Pezhouman, Ngoc B. Nguyen, Allison Shevtsov, Rong Qiao, Reza Ardehali
      Pages 257-267
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Keywords

About this book

This volume explores the fields of stem cell biology, regenerative medicine, and cancer biology. The chapters in this book cover topics such as embryonic stem cells (ES) and induced pluripotent cells (iPS); ES/iPSC derived tissue stem cells; tissue resident stem cells; assays for primitive malignant cells; and CRISPR/Cas9 engineering and transgenic model development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and thorough, Stem Cell Assays: Methods and Protocols is a valuable laboratory manual that allows researchers and scientists to learn about new techniques and approaches in this developing field.


Editors and Affiliations

  • Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, USA

    Nagarajan Kannan

  • Hull York Medical School, York, UK

    Philip Beer

Bibliographic Information

  • Book Title: Stem Cell Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Nagarajan Kannan, Philip Beer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1979-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1978-0Published: 05 May 2022

  • Softcover ISBN: 978-1-0716-1981-0Published: 06 May 2023

  • eBook ISBN: 978-1-0716-1979-7Published: 04 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 569

  • Number of Illustrations: 31 b/w illustrations, 92 illustrations in colour

  • Topics: Stem Cells

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