Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2398)
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Table of contents (19 protocols)
Keywords
About this book
This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.
“Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
Bibliographic Information
Book Title: Plant Circadian Networks
Book Subtitle: Methods and Protocols
Editors: Dorothee Staiger, Seth Davis, Amanda Melaragno Davis
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1912-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1911-7Published: 22 October 2021
Softcover ISBN: 978-1-0716-1914-8Published: 23 October 2022
eBook ISBN: 978-1-0716-1912-4Published: 21 October 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 245
Number of Illustrations: 17 b/w illustrations, 33 illustrations in colour
Topics: Plant Sciences