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Bacterial Persistence

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Natalie Verstraeten

    1. VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Jan Michiels

    1. VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocol
  • Provides step-by-step detail essential for reproducible result

Part of the book series: Methods in Molecular Biology (MIMB, volume 2357)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Studying Bacterial Persistence: Established Methods and Current Advances

      • Elen Louwagie, Laure Verstraete, Jan Michiels, Natalie Verstraeten
      Pages 3-20

  3. Quantification and Enrichment of Persister Cells

    1. Front Matter

      Pages 21-21
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    2. Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases

      • Enea Maffei, Cinzia Fino, Alexander Harms
      Pages 23-40

    3. A Robust Method for Generating, Quantifying, and Testing Large Numbers of Escherichia coli Persisters

      • Silke R. Vedelaar, Jakub L. Radzikowski, Matthias Heinemann
      Pages 41-62

    4. Enrichment of Persister Cells Through Β-Lactam-Induced Filamentation and Size Separation

      • Etthel Windels, Bram Van den Bergh, Jan Michiels
      Pages 63-69

    5. Methods for Enrichment of Bacterial Persister Populations for Phenotypic Screens and Genomic Studies

      • Samantha Adikari, Elizabeth Hong-Geller, Sofiya Micheva-Viteva
      Pages 71-82

  4. Single-Cell Analysis of Persister Cells

    1. Front Matter

      Pages 83-83
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    2. Observing Bacterial Persistence at Single-Cell Resolution

      • Emma Dawson, Emrah Şimşek, Minsu Kim
      Pages 85-93

    3. Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis

      • Nathan Fraikin, Laurence Van Melderen, Frédéric Goormaghtigh
      Pages 95-106

    4. Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation

      • Stefany Moreno-Gámez, Alma Dal Co, Simon van Vliet, Martin Ackermann
      Pages 107-124

    5. Counting Chromosomes in Individual Bacteria to Quantify Their Impacts on Persistence

      • Allison M. Murawski, Katherine Rittenbach, Christina J. DeCoste, Gary Laevsky, Mark P. Brynildsen
      Pages 125-146

  5. Omics Approaches in Persistence Research

    1. Front Matter

      Pages 147-147
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    2. Analyzing Persister Proteomes with SILAC and Label-Free Methods

      • Bork A. Berghoff
      Pages 149-159

    3. Defining Proteomic Signatures to Predict Multidrug Persistence in Pseudomonas aeruginosa

      • Pablo Manfredi, Isabella Santi, Enea Maffei, Emmanuelle Lezan, Alexander Schmidt, Urs Jenal
      Pages 161-175

    4. The Use of Experimental Evolution to Study the Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment

      • Isabella Santi, Pablo Manfredi, Urs Jenal
      Pages 177-194

  6. Persister Cell Resuscitation and Eradication

    1. Front Matter

      Pages 195-195
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    2. Detecting Persister Awakening Determinants

      • Dorien Wilmaerts, Jan Michiels, Natalie Verstraeten
      Pages 197-208

    3. Monitoring Persister Resuscitation with Flow Cytometry

      • Sayed Golam Mohiuddin, Mehmet A. Orman
      Pages 209-222
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Keywords

About this book

This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.

Editors and Affiliations

  • VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium

    Natalie Verstraeten, Jan Michiels

Bibliographic Information

  • Book Title: Bacterial Persistence

  • Book Subtitle: Methods and Protocols

  • Editors: Natalie Verstraeten, Jan Michiels

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1621-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1620-8Published: 30 September 2021

  • Softcover ISBN: 978-1-0716-1623-9Published: 01 October 2022

  • eBook ISBN: 978-1-0716-1621-5Published: 29 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 292

  • Number of Illustrations: 9 b/w illustrations, 42 illustrations in colour

  • Topics: Immunology, Bacteriology

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