Skip to main content
  • Book
  • © 2021

Analytical Methodologies for Biofilm Research

  • Provides step-by-step, easy to follow methodologies in biofilm research, includes relevant case studies
  • Discusses the latest analytical methods to study biofilms and disease diagnosis
  • Provides diagram, graphs, troubleshooting tips, to enable readers to replicate the experiments

Part of the book series: Springer Protocols Handbooks (SPH)

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (14 protocols)

  1. Front Matter

    Pages i-vii
  2. Introduction to Biofilms

    1. Front Matter

      Pages 1-1
    2. Biofilm Formation and Pathogenesis

      • Ankurita Bhowmik, Akshit Malhotra, Sangita Jana, Ashwini Chauhan
      Pages 3-37
  3. Separation Methods and Spectroscopic Techniques in Biofilm Research

    1. Front Matter

      Pages 67-67
    2. Analysing Microbial Biofilm Formation at a Molecular Level: Role of Fourier Transform Infrared and Raman Spectroscopy

      • Moupriya Nag, Dibyajit Lahiri, Ritwik Banerjee, Abarna Chatterjee, Anushka Ghosh, Prateek Banerjee et al.
      Pages 69-93
    3. In Vitro Metabolite Profiling of Microbial Biofilm: Role of Gas Chromatography and High-Performance Liquid Chromatography

      • Dibyajit Lahiri, Moupriya Nag, Deboleena Das, Sushantika Chatterjee, Ankita Dey, Rina Rani Ray
      Pages 95-113
  4. Electroanalytical Techniques for Evaluation of Biofilm Research

    1. Front Matter

      Pages 115-115
    2. Contribution of Cyclic Voltammetry and Electrochemical Impedance Spectroscopy in Deciphering the Electron Transport System in Biofilm

      • Sushma Dave, Jayashankar Das, Shivani Dave, Padmaja Mohanty, G. M. Priya, Pratik Jagtap et al.
      Pages 129-152
  5. In Situ Surface Analysis and Imaging Techniques in Biofilm Research

    1. Front Matter

      Pages 181-181
    2. Imaging Bacteria and Biofilm by Field Emission Scanning Electron Microscopy

      • Kannappan Arunachalam, MubarakAli Davoodbasha
      Pages 205-222
    3. Probing the Surface-Attached In Vitro Microbial Biofilms with Atomic Force (AFM) and Scanning Probe Microscopy (SPM)

      • Sudipta Dash, Dibyajit Lahiri, Moupriya Nag, Deboleena Das, Rina Rani Ray
      Pages 223-241
  6. Molecular Biology and Recombinant DNA Techniques in Biofilm Research

    1. Front Matter

      Pages 267-267
    2. Genetic Basis of Biofilm Formation and Spread of Nosocomial Infections

      • Snehashis Koley, Mandira Mukherjee
      Pages 269-298
    3. Monitoring Cell Distribution and Death in Sessile Forms of Microbial Biofilm: Flow Cytometry-Fluorescence Activated Cell Sorting (FCM-FACS)

      • Dipro Mukherjee, Sayantani Garai, Dibyajit Lahiri, Moupriya Nag, Rina Rani Ray
      Pages 299-316

About this book

The book provides the readers of various discipline an easy understanding of the latest biophysical techniques pertaining to microbiology. Biofilm associated chronic infection is a major health problem and a serious concern to doctors, scientists and other health workers as it develops antibiotic and multi-drug resistance. This book describes various protocols utilized in the detection of the biofilm. The book has been divided into six sub sections which provides pertinent information about the various biophysical techniques and instruments that are used for detecting and analyzing the biofilm formation upon biotic and abiotic surfaces. The readers will be able to identify the techniques that can best cater information to solve the problem at hand. This book attempts to compile the latest information on the recent advances in the various functional aspects of microbial biofilms, their pathogenesis, present day treatments as well as detection strategies.

This book is meant forresearchers in the field of microbiology and interested in understanding microbial pathogenesis, quorum sensing and biofilm formation.



Editors and Affiliations

  • Department of Biotechnology, University of Engineering & Management, Kolkata, India

    Moupriya Nag, Dibyajit Lahiri

About the editors

Moupriya Nag is presently working as Assistant Professor in the Department of Biotechnology, University of Engineering & Management, Kolkata. Her research interests includes single molecule Biophysics of protein unfolding/refolding and protein aggregation kinetics and its mechanism of action especially the amyloid proteins in neurodegenerative disorders.

Recently, her research focuses on functional amyloids in bacteria and their relation in forming biofilms. Her work deals with developing novel antimicrobial and anti-biofilm compounds from natural sources including plants, microbes and green synthesized nanoparticles.
She has about five years of teaching experience in Biophysics, Bioinformatics,
Bioprocess and Biotechnology, Enzyme Technology, Computing, Molecular Modeling and Drug Design. She has also published many research articles in the peer-reviewed international journal and authored or co-authored numerous book chapters. She is also amember of many scientific societies like Indian Biophysical Society and Indian Science Congress Association


Dibyajit Lahiri is at present is working as Assistant Professor in the Department of Biotechnology, University of Engineering & Management, Kolkata.  His research interest is precisely on biofilm isolated from human prosthesis and its inhibition by various novel phyto and nano compounds. He is keen to explore the molecular mechanism behind the removal of biofilm by natural compounds. His research arena also encompasses computational drug development using bioinformatics. His work is regularly being presented and appreciated before a number of experts of National and International repute. He is a life Member of Indian Science Congress Association and Institution of Engineers (India).





Bibliographic Information

  • Book Title: Analytical Methodologies for Biofilm Research

  • Editors: Moupriya Nag, Dibyajit Lahiri

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-1378-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1377-1Published: 10 July 2021

  • Softcover ISBN: 978-1-0716-1380-1Published: 10 July 2022

  • eBook ISBN: 978-1-0716-1378-8Published: 09 July 2021

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: VII, 343

  • Number of Illustrations: 24 b/w illustrations, 32 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access