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Next Generation Culture Platforms for Reliable In Vitro Models

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Tiziana A.L. Brevini

    1. Laboratory of Biomedical Embryology, Università degli Studi di Milano, Milan, Italy

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  2. Alireza Fazeli

    1. Department of Pathophysiology, University of Tartu, Tartu, Estonia

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    You can also search for this editor in PubMed   Google Scholar

  3. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step details essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2273)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

    • Alberto Maria Luciano, Rodrigo Garcia Barros, Ana Caroline Silva Soares, Jose Buratini, Valentina Lodde, Federica Franciosi
    Pages 1-15

  3. Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids

    • Krzysztof Wrzesinski, Helle Sedighi Frandsen, Carlemi Calitz, Chrisna Gouws, Barbara Korzeniowska, Stephen J. Fey
    Pages 17-62

  4. Protocol to Study the Role of Extracellular Vesicles During Induced Stem Cell Differentiation

    • Kelly C. S. Roballo, Carlos E. Ambrosio, Juliano C. da Silveira
    Pages 63-73

  5. A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors

    • Malgorzata Duda, Lucia Gizler, Gabriela Gorczyca
    Pages 75-84

  6. Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres

    • Jeffrey Aalders, Laurens Léger, Davide Piras, Jolanda van Hengel, Sergio Ledda
    Pages 85-102

  7. Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models

    • Antonio Murillo, Marta Muñoz
    Pages 103-110

  8. Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues

    • Matteo Ghiringhelli, Yousef Abboud, Snizhanna V. Chorna, Irit Huber, Gil Arbel, Amira Gepstein et al.
    Pages 111-129

  9. Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models

    • Georgia Pennarossa, Alireza Fazeli, Sergio Ledda, Fulvio Gandolfi, Tiziana A. L. Brevini
    Pages 131-138

  10. Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bioscaffold

    • Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, Tiziana A. L. Brevini
    Pages 139-149

  11. A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells

    • Sharon Arcuri, Fulvio Gandolfi, Edgardo Somigliana, Tiziana A. L. Brevini
    Pages 151-158

  12. 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids

    • Joel Mario Vej-Nielsen, Adelina Rogowska-Wrzesinska
    Pages 159-171

  13. Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics

    • Helle Sedighi Frandsen, Martina Štampar, Joel Mario Vej-Nielsen, Bojana Žegura, Adelina Rogowska-Wrzesinska
    Pages 173-188

  14. Isolation of Extracellular Vesicles (EVs) Using Size-Exclusion High-Performance Liquid Chromatography (SE-HPLC)

    • Keerthie Dissanayake, Kasun Godakumara, Alireza Fazeli
    Pages 189-200

  15. Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns

    • Qurat Ul Ain Reshi, Mohammad Mehedi Hasan, Keerthie Dissanayake, Alireza Fazeli
    Pages 201-206

  16. Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer

    • Keerthie Dissanayake, Getnet Midekessa, Freddy Lättekivi, Alireza Fazeli
    Pages 207-218

  17. Isolation, Characterization, and MicroRNA Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids

    • Karina Cañón-Beltrán, Meriem Hamdi, Rosane Mazzarella, Yulia N. Cajas, Claudia L. V. Leal, Alfonso Gutiérrez-Adán et al.
    Pages 219-238

  18. Biomimetic 3D-Bone Tissue Model

    • Mahmut Parmaksiz, Ayşe Eser Elçin, Yaşar Murat Elçin
    Pages 239-250

  19. Using the Air–Liquid Interface Approach to Foster Apical–Basal Polarization of Mammalian Female Reproductive Tract Epithelia In Vitro

    • Shuai Chen, Jennifer Schoen
    Pages 251-262

  20. Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa

    • Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A. L. Brevini, Sandra Van Vlierberghe, Fulvio Gandolfi
    Pages 263-278
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Keywords

About this book

This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.

Editors and Affiliations

  • Laboratory of Biomedical Embryology, Università degli Studi di Milano, Milan, Italy

    Tiziana A.L. Brevini

  • Department of Pathophysiology, University of Tartu, Tartu, Estonia

    Alireza Fazeli

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Next Generation Culture Platforms for Reliable In Vitro Models

  • Book Subtitle: Methods and Protocols

  • Editors: Tiziana A.L. Brevini, Alireza Fazeli, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1246-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1245-3Published: 19 February 2021

  • Softcover ISBN: 978-1-0716-1248-4Published: 19 February 2022

  • eBook ISBN: 978-1-0716-1246-0Published: 18 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 307

  • Number of Illustrations: 10 b/w illustrations, 59 illustrations in colour

  • Topics: Developmental Biology, Regenerative Medicine/Tissue Engineering

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